ArticleWorld journal of gastroenterology2005
Effects of different ingredients of zedoary on gene expression of HSC-T6 cells.
Article in World journal of gastroenterology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 46 citations in OpenAlex.
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- Curcumol inhibits the proliferation and metastasis of melanoma via the miR-152-3p/PI3K/AKT and ERK/NF-κB signaling pathways.Journal of Cancer · 2020Article
- The role of curcumin in liver diseases.Archives of medical science : AMS · 2019Article
- Curcumol: From Plant Roots to Cancer Roots.International journal of biological sciences · 2019Review
- Curcumol attenuates epithelial-mesenchymal transition of nasopharyngeal carcinoma cells via TGF-β1.Molecular medicine reports · 2018Article
- Pharmacokinetic Comparisons of Typical Constituents inEvidence-based complementary and alternative medicine : eCAM · 2018Article
- Curcumol potentiates celecoxib-induced growth inhibition and apoptosis in human non-small cell lung cancer.Oncotarget · 2017Article
- Curcumol Promotes Vascular Endothelial Growth Factor (VEGF)-Mediated Diabetic Wound Healing in Streptozotocin-Induced Hyperglycemic Rats.Medical science monitor : international medical journal of experimental and clinical research · 2017Article
- cDNA Microarray Analysis Revealing Candidate Biomineralization Genes of the Pearl Oyster, Pinctada fucata martensii.Marine biotechnology (New York, N.Y.) · 2016Article
- Curcumol Inhibits Growth and Induces Apoptosis of Colorectal Cancer LoVo Cell Line via IGF-1R and p38 MAPK Pathway.International journal of molecular sciences · 2015Article
- Curcumin targets multiple pathways to halt hepatic stellate cell activation: updated mechanisms in vitro and in vivo.Digestive diseases and sciences · 2015Review
- RNA interference against discoidin domain receptor 2 ameliorates alcoholic liver disease in rats.PloS one · 2013Article
- The use of transcriptomics to unveil the role of nutrients in Mammalian liver.ISRN nutrition · 2013Review
- Curcuma Contra Cancer? Curcumin and Hodgkin's Lymphoma.Cancer growth and metastasis · 2013Review
- Inhibitory Effect of Curcumol on Jak2-STAT Signal Pathway Molecules of Fibroblast-Like Synoviocytes in Patients with Rheumatoid Arthritis.Evidence-based complementary and alternative medicine : eCAM · 2012Article
- TCMGeneDIT: a database for associated traditional Chinese medicine, gene and disease information using text mining.BMC complementary and alternative medicine · 2008Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo investigate the effects of four different ingredients of zedoary (Curcuma aromatica oil, Curcumol, beta-elemence, and Curcumin) on the gene expressions of hepatic stellate cells (HSCs), and to explore the molecular mechanism of zedoary against hepatic fibrosis at gene network level.
methodsWe detected the mRNA sequences of 50 liver fibrosis-related genes in GenBank and designed oligonucleotide probes. We synthesized oligonucleotides with PE8909 DNA synthesizing instrument, and carried out oligonucleotide microarray with OGR-04 dropping instrument and aldehyded glass chip. Cultured HSC-T6 cells were treated with different concentrations of Colchicine, Curcuma aromatica oil, Curcumol, beta-elemence, and Curcumin. According to the experiment of cell toxicity, we took the appropriate concentrations of medicines that resulted in over 50% of cell survival as experiment concentrations. We collected the cells at 1, 6, 12, and 24 h, and extracted total RNA with TRIzol reagent, then labeled cDNAs with Cy3-dUTP and Cy5-dUTP. These labeled cDNAs were hybridized to an oligonucleotide microarray which was washed several times and scanned by scanner GenePix 4000B. Different gene expressions of HSC-T6 cells were analyzed by ImaGene 4.2 software.
resultsAfter HSC-T6 cells were cultured in a medium containing 6.25 microg/mL Colchicine for 12 h, expression of TIMP-1 decreased 2.2-folds. After HSC-T6 cells were cultured in a medium containing 78.125 microg/mL of Curcuma aromatica oil for 24 h, the expression of TIMP-2 and IL-6 decreased 2.3- and 2.2-folds, respectively. Moreover, after HSC-T6 cells were cultured in a medium containing 1.5625 microg/mL of Curcumol for 12 h, the expression of TGFbeta1 and P450a decreased 2.3- and 2.1-folds, respectively.
conclusionOur results may show the possible molecular mechanism of Curcuma aromatica oil and Curcumol against hepatic fibrosis.
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