ArticlePlanta2006
High levels of asparagine synthetase in hypocotyls of pine seedlings suggest a role of the enzyme in re-allocation of seed-stored nitrogen.
Article in Planta, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 54 citations in OpenAlex.
- Expansion and Nitrate-Responsive Expression of NRT3 Transport Regulators in Maritime Pine.Plants (Basel, Switzerland) · 2026Article
- The Multifaceted Functions of Plant Asparagine Synthetase: Regulatory Mechanisms and Functional Diversity in Growth and Defense.Plants (Basel, Switzerland) · 2026Review
- Metabolic network changes during skotomorphogenesis inPlant direct · 2022Article
- Epitranscriptome changes triggered by ammonium nutrition regulate the proteome response of maritime pine roots.Frontiers in plant science · 2022Article
- Inorganic Nitrogen Form Determines Nutrient Allocation and Metabolic Responses in Maritime Pine Seedlings.Plants (Basel, Switzerland) · 2020Article
- Establishing gene models from the Pinus pinaster genome using gene capture and BAC sequencing.BMC genomics · 2016Article
- Reassimilation of Photorespiratory Ammonium in Lotus japonicus Plants Deficient in Plastidic Glutamine Synthetase.PloS one · 2015Article
- Evolution of gene structure in the conifer Picea glauca: a comparative analysis of the impact of intron size.BMC plant biology · 2014Article
- Arabidopsis plastidial folylpolyglutamate synthetase is required for seed reserve accumulation and seedling establishment in darkness.PloS one · 2014Article
- Novel insights into regulation of asparagine synthetase in conifers.Frontiers in plant science · 2012Article
- Carbon allocation in ectomycorrhizal plants at limited optimal N supply: an attempt aat unraveling conflicting theories.Mycorrhiza · 2011Article
- Molecular and functional analyses support a role of Ornithine-{delta}-aminotransferase in the provision of glutamate for glutamine biosynthesis during pine germination.Plant physiology · 2008Article
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A pine asparagine synthetase gene expressed in developing seedlings has been identified by cloning its cDNA (PsAS1) from Scots pine (Pinus sylvestris L.). Genomic DNA analysis with PsAS1 probes and a sequence-based phylogenetic tree are consistent with the possibility of more than one gene encoding asparagine synthetase in pine. However, the parallel patterns of free asparagine content and PsAS1 products indicate that the protein encoded by this gene is mainly responsible for the accumulation of this amino acid during germination and early seedling development. The temporal and spatial patterns of PsAS1 expression together with the spatial distribution of asparagine content suggest that, early after germination, part of the nitrogen mobilized from the megagametophyte is diverted toward the hypocotyl to produce high levels of asparagine as a reservoir of nitrogen to meet later specific demands of development. Furthermore, the transcript and protein analyses in seedlings germinated and growth for extended periods under continuous light or dark suggest that the spatial expression pattern of PsAS1 is largely determined by a developmental program. Therefore, our results suggest that the spatial and temporal control of PsAS1 expression determines the re-allocation of an important amount of seed-stored nitrogen during pine germination.
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