ArticleThe EMBO journal2006
Cell-cycle-dependent Xenopus TRF1 recruitment to telomere chromatin regulated by Polo-like kinase.
Article in The EMBO journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- CDK1 dependent phosphorylation of hTERT contributes to cancer progression.Nature communications · 2020Article
- Phylointeractomics reconstructs functional evolution of protein binding.Nature communications · 2017Article
- Resolution of telomere associations by TRF1 cleavage in mouse embryonic stem cells.Molecular biology of the cell · 2014Article
- The 68-kDa telomeric repeat binding factor 1 (TRF1)-associated protein (TAP68) interacts with and recruits TRF1 to the spindle pole during mitosis.The Journal of biological chemistry · 2014Article
- Involvement of telomerase reverse transcriptase in heterochromatin maintenance.Molecular and cellular biology · 2014Article
- Xenopus DNA2 is a helicase/nuclease that is found in complexes with replication proteins And-1/Ctf4 and Mcm10 and DSB response proteins Nbs1 and ATM.Cell cycle (Georgetown, Tex.) · 2010Article
- Origin-dependent initiation of DNA replication within telomeric sequences.Nucleic acids research · 2010Article
- GNL3L stabilizes the TRF1 complex and promotes mitotic transition.The Journal of cell biology · 2009Article
- Ubiquitin Ligase RLIM Modulates Telomere Length Homeostasis through a Proteolysis of TRF1.The Journal of biological chemistry · 2009Article
- Plk1 phosphorylation of TRF1 is essential for its binding to telomeres.The Journal of biological chemistry · 2008Article
- Telomere dynamics: the means to an end.Cell proliferation · 2007Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Telomeres are regulated by a homeostatic mechanism that includes telomerase and telomeric repeat binding proteins, TRF1 and TRF2. Recently, it has been hypothesized that telomeres assume distinct configurations in a cell-cycle-dependent manner, although direct biochemical evidence is lacking. Here we demonstrated that Xenopus TRF1 (xTRF1) associates with telomere chromatin specifically in mitotic Xenopus egg extracts, and dissociates from it upon mitotic exit. Both the N-terminal TRF-homology (TRFH) domain and the linker region connecting the TRFH domain and the C-terminal Myb domain are required for this cell-cycle-dependent association of xTRF1 with chromatin. In contrast, Xenopus TRF2 (xTRF2) associates with chromatin throughout the cell cycle. We showed that Polo-like kinase (Plx1) phosphorylates xTRF1 in vitro. Moreover, the mitotic xTRF1-chromatin association was significantly impaired when Plx1 was immunodepleted from the extracts. Finally, high telomerase activities were detected in association with replicating interphase chromatin compared with mitotic chromatin. These results indicate that telomere chromatin is actively regulated by cell-cycle-dependent processes, and provide an insight for understanding how telomeres undergo DNA metabolisms during the cell cycle.
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