ArticleThe Journal of physiology2006
A possible role of the junctional face protein JP-45 in modulating Ca2+ release in skeletal muscle.
Article in The Journal of physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- Architecture and molecular machinery of skeletal myofibers: a systematic review of the structure-function relationships.Frontiers in cell and developmental biology · 2025Review
- Calsequestrin: a well-known but curious protein in skeletal muscle.Experimental & molecular medicine · 2020Review
- CaCells · 2019Review
- Organization of junctional sarcoplasmic reticulum proteins in skeletal muscle fibers.Journal of muscle research and cell motility · 2015Review
- The couplonopathies: A comparative approach to a class of diseases of skeletal and cardiac muscle.The Journal of general physiology · 2015Review
- JP-45/JSRP1 variants affect skeletal muscle excitation-contraction coupling by decreasing the sensitivity of the dihydropyridine receptor.Human mutation · 2013Article
- The role of CACNA1S in predisposition to malignant hyperthermia.BMC medical genetics · 2009Article
- Minor sarcoplasmic reticulum membrane components that modulate excitation-contraction coupling in striated muscles.The Journal of physiology · 2009Review
- Evolution and modulation of intracellular calcium release during long-lasting, depleting depolarization in mouse muscle.The Journal of physiology · 2008Article
- The calcium channel alpha2/delta1 subunit is involved in extracellular signalling.The Journal of physiology · 2008Article
- Loss of skeletal muscle strength by ablation of the sarcoplasmic reticulum protein JP45.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- A musculoskeletal model of low grade connective tissue inflammation in patients with thyroid associated ophthalmopathy (TAO): the WOMED concept of lateral tension and its general implications in disease.BMC musculoskeletal disorders · 2007Article
- Bridging the myoplasmic gap: recent developments in skeletal muscle excitation-contraction coupling.Journal of muscle research and cell motility · 2007Review
- Calcium signalling in muscle: a milestone for modulation studies.The Journal of physiology · 2006Article
- Novel regulators of RyR Ca2+ release channels: insight into molecular changes in genetically-linked myopathies.Journal of muscle research and cell motility · 2006Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the functional role of JP-45, a recently discovered protein of the junctional face membrane (JFM) of skeletal muscle. For this purpose, we expressed JP-45 C-terminally tagged with the fluorescent protein DsRed2 by nuclear microinjection in myotubes derived from the C2C12 skeletal muscle cell line and performed whole-cell voltage-clamp experiments. We recorded in parallel cell membrane currents and Ca(2+) signals using fura-2 during step depolarization. It was found that properties of the voltage-activated Ca(2+) current were not significantly changed in JP-45-DsRed2-expressing C2C12 myotubes whereas the amplitude of depolarization-induced Ca(2+) transient was decreased compared to control myotubes expressing only DsRed2. Converting Ca(2+) transients to Ca(2+) input flux using a model fit approach to quantify Ca(2+) removal, the change could be attributed to an alteration in voltage-activated Ca(2+) permeability rather than to altered removal properties or a lower Ca(2+) content of the sarcoplasmic reticulum (SR). Determining non-linear capacitive currents revealed a reduction of Ca(2+) permeability per voltage-sensor charge. The results may be explained by a modulatory effect of JP-45 related to its reported in vitro interaction with the dihydropyridine receptor and the SR Ca(2+) binding protein calsequestrin (CSQ).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.