ArticleNucleic acids research2006
Sub-plastidial localization of two different phage-type RNA polymerases in spinach chloroplasts.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 23 citations in OpenAlex.
- Cellular roadmaps of viroid infection.Trends in microbiology · 2023Review
- Plastid gene expression during chloroplast differentiation and dedifferentiation into non-photosynthetic plastids during seed formation.Plant molecular biology · 2013Article
- How to build functional thylakoid membranes: from plastid transcription to protein complex assembly.Planta · 2013Review
- Function of plastid sigma factors in higher plants: regulation of gene expression or just preservation of constitutive transcription?Plant molecular biology · 2011Article
- Intraplastidial trafficking of a phage-type RNA polymerase is mediated by a thylakoid RING-H2 protein.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Impaired function of the phage-type RNA polymerase RpoTp in transcription of chloroplast genes is compensated by a second phage-type RNA polymerase.Nucleic acids research · 2008Article
- Arabidopsis phage-type RNA polymerases: accurate in vitro transcription of organellar genes.The Plant cell · 2007Article
- Building up of the plastid transcriptional machinery during germination and early plant development.Plant physiology · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant plastids contain a circular genome of approximately 150 kb organized into approximately 35 transcription units. The plastid genome is organized into nucleoids and attached to plastid membranes. This relatively small genome is transcribed by at least two different RNA polymerases, one being of the prokaryotic type and plastid-encoded (PEP), the other one being of the phage-type and nucleus-encoded (NEP). The presumed localization of a second phage-type RNA polymerase in plastids is still questionable. There is strong evidence for a sequential action of NEP and PEP enzymes during plant development attributing a prevailing role of NEP during early plant and plastid development, although NEP is present in mature chloroplasts. In the present paper, we have analysed two different NEP enzymes from spinach with respect to subcellular and intra-plastidial localization in mature chloroplasts with the help of specific antibodies. Results show the presence of the two different NEP enzymes in mature chloroplasts. Both enzymes are entirely membrane bound but, unlike previously thought, this membrane binding is not mediated via DNA. This finding indicates that NEP enzymes are not found as elongating transcription complexes on the template DNA in mature chloroplasts and raises the question of their function in mature chloroplasts.
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