ArticleNucleic acids research2006
Genetic manipulation of an exogenous non-immunoglobulin protein by gene conversion machinery in a chicken B cell line.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- Molecular mechanisms of avian immunoglobulin gene diversification and prospect for industrial applications.Frontiers in immunology · 2024Review
- Streamlined human antibody generation and optimization by exploiting designed immunoglobulin loci in a B cell line.Cellular & molecular immunology · 2021Article
- Article
- Harnessing gene conversion in chicken B cells to create a human antibody sequence repertoire.PloS one · 2013Article
- Activation-induced cytidine deaminase (AID)-dependent somatic hypermutation requires a splice isoform of the serine/arginine-rich (SR) protein SRSF1.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Directed evolution of mammalian anti-apoptosis proteins by somatic hypermutation.Protein engineering, design & selection : PEDS · 2012Article
- Technologies of directed protein evolution in vivo.Cellular and molecular life sciences : CMLS · 2011Review
- Protein and genome evolution in Mammalian cells for biotechnology applications.Molecular biotechnology · 2009Review
- Green fluorescent protein is superior to blue fluorescent protein as a quantitative reporter of promoter activity in E. coli.Molecular biology reports · 2009Article
- Activation-induced cytidine deaminase-mediated hypermutation in the DT40 cell line.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2009Review
- Protein evolution by hypermutation and selection in the B cell line DT40.Nucleic acids research · 2008Article
- Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During culture, a chicken B cell line DT40 spontaneously mutates immunoglobulin (Ig) genes by gene conversion, which involves activation-induced cytidine deaminase (AID)-dependent homologous recombination of the variable (V) region gene with upstream pseudo-V genes. To explore whether this mutation mechanism can target exogenous non-Ig genes, we generated DT40 lines that bears a gene conversion substrate comprising the green fluorescent protein (GFP) gene as a donor and the blue fluorescent protein (BFP) gene as an acceptor. A few percent of the initially BFP-expressing cells converted their fluorescence from blue to green after culture for 2-3 weeks when the substrate construct was integrated in the Ig light chain locus, but not in the ovalbumin locus. This was the result of AID-dependent and the GFP gene-templated gene conversion of the BFP gene, thereby leading to the introduction of various sizes of GFP-derived gene segment into the BFP gene. Thus, G/B construct may be used to visualize gene conversion events. After switching off AID expression in DT40 cells, the mutant clones were isolated stably and maintained with their mutations being fixed. Thus, the gene conversion machinery in DT40 cells will be a useful means to engineer non-Ig proteins by a type of DNA shuffling.
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