ArticleMolecular biology of the cell2006
The F-box protein Dia2 regulates DNA replication.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 47 citations in OpenAlex.
- sic1 mutation leads to rDNA instability by partial duplication with SIR4.Nucleic acids research · 2026Article
- SKP1-CUL1-F-box: Key molecular targets affecting disease progression.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Review
- CMG helicase disassembly is essential and driven by two pathways in budding yeast.The EMBO journal · 2024Article
- The Effect of Dia2 Protein Deficiency on the Cell Cycle, Cell Size, and Recruitment of Ctf4 Protein inMolecules (Basel, Switzerland) · 2021Article
- Mechanisms of eukaryotic replisome disassembly.Biochemical Society transactions · 2020Review
- Chromatin-Bound Cullin-Ring Ligases: Regulatory Roles in DNA Replication and Potential Targeting for Cancer Therapy.Frontiers in molecular biosciences · 2018Review
- Mechanisms of DNA replication termination.Nature reviews. Molecular cell biology · 2017Review
- Degradation of Mrc1 promotes recombination-mediated restart of stalled replication forks.Nucleic acids research · 2017Article
- CRL2Genes & development · 2017Article
- Isolation of ubiquitinated substrates by tandem affinity purification of E3 ligase-polyubiquitin-binding domain fusions (ligase traps).Nature protocols · 2016Article
- Analysis of the Histone H3.1 Interactome: A Suitable Chaperone for the Right Event.Molecular cell · 2015Article
- Tethering of SCF(Dia2) to the Replisome Promotes Efficient Ubiquitylation and Disassembly of the CMG Helicase.Current biology : CB · 2015Article
- Review
- Cdc48 and a ubiquitin ligase drive disassembly of the CMG helicase at the end of DNA replication.Science (New York, N.Y.) · 2014Article
- The subunits of the S-phase checkpoint complex Mrc1/Tof1/Csm3: dynamics and interdependence.Cell division · 2014Article
- Proteasome-dependent degradation of replisome components regulates faithful DNA replication.Cell cycle (Georgetown, Tex.) · 2013Review
- The Saccharomyces cerevisiae F-box protein Dia2 is a mediator of S-phase checkpoint recovery from DNA damage.Genetics · 2013Article
- Coordinated degradation of replisome components ensures genome stability upon replication stress in the absence of the replication fork protection complex.PLoS genetics · 2013Article
- The Hect domain E3 ligase Tom1 and the F-box protein Dia2 control Cdc6 degradation in G1 phase.The Journal of biological chemistry · 2012Article
- Hect E3 ubiquitin ligase Tom1 controls Dia2 degradation during the cell cycle.Molecular biology of the cell · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ubiquitin-mediated proteolysis plays a key role in many pathways inside the cell and is particularly important in regulating cell cycle transitions. SCF (Skp1/Cul1/F-box protein) complexes are modular ubiquitin ligases whose specificity is determined by a substrate-binding F-box protein. Dia2 is a Saccharomyces cerevisiae F-box protein previously described to play a role in invasive growth and pheromone response pathways. We find that deletion of DIA2 renders cells cold-sensitive and subject to defects in cell cycle progression, including premature S-phase entry. Consistent with a role in regulating DNA replication, the Dia2 protein binds replication origins. Furthermore, the dia2 mutant accumulates DNA damage in both S and G2/M phases of the cell cycle. These defects are likely a result of the absence of SCF(Dia2) activity, as a Dia2 DeltaF-box mutant shows similar phenotypes. Interestingly, prolonging G1-phase in dia2 cells prevents the accumulation of DNA damage in S-phase. We propose that Dia2 is an origin-binding protein that plays a role in regulating DNA replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.