ArticleMolecular biology of the cell2006
A genetic dissection of Aip1p's interactions leads to a model for Aip1p-cofilin cooperative activities.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 40 citations in OpenAlex.
- Mechanisms of actin disassembly and turnover.The Journal of cell biology · 2023Review
- Catastrophic actin filament bursting by cofilin, Aip1, and coronin.The Journal of biological chemistry · 2020Article
- Functions of actin-interacting protein 1 (AIP1)/WD repeat protein 1 (WDR1) in actin filament dynamics and cytoskeletal regulation.Biochemical and biophysical research communications · 2018Review
- Phosphomimetic S3D cofilin binds but only weakly severs actin filaments.The Journal of biological chemistry · 2017Article
- Actin-interacting Protein 1 Promotes Disassembly of Actin-depolymerizing Factor/Cofilin-bound Actin Filaments in a pH-dependent Manner.The Journal of biological chemistry · 2016Article
- Actin Mechanics and Fragmentation.The Journal of biological chemistry · 2015Review
- Actin and endocytosis in budding yeast.Genetics · 2015Review
- Aip1 promotes actin filament severing by cofilin and regulates constriction of the cytokinetic contractile ring.The Journal of biological chemistry · 2015Article
- Aip1 destabilizes cofilin-saturated actin filaments by severing and accelerating monomer dissociation from ends.Current biology : CB · 2014Article
- Linking genetics to structural biology: complex heterozygosity screening with actin alanine scan alleles identifies functionally related surfaces on yeast actin.G3 (Bethesda, Md.) · 2014Article
- Coordination of the filament stabilizing versus destabilizing activities of cofilin through its secondary binding site on actin.Cytoskeleton (Hoboken, N.J.) · 2014Article
- Cyclase-associated protein (CAP) acts directly on F-actin to accelerate cofilin-mediated actin severing across the range of physiological pH.The Journal of biological chemistry · 2012Article
- The two actin-interacting protein 1 genes have overlapping and essential function for embryonic development in Caenorhabditis elegans.Molecular biology of the cell · 2011Article
- Diverse protective roles of the actin cytoskeleton during oxidative stress.Cytoskeleton (Hoboken, N.J.) · 2011Article
- Expression of actin-interacting protein 1 suppresses impaired chemotaxis of Dictyostelium cells lacking the Na+-H+ exchanger NHE1.Molecular biology of the cell · 2010Article
- Overlapping and distinct functions for cofilin, coronin and Aip1 in actin dynamics in vivo.Journal of cell science · 2010Article
- Unbalancing the phosphatidylinositol-4,5-bisphosphate-cofilin interaction impairs cell steering.Molecular biology of the cell · 2009Article
- Actin-ADF/cofilin rod formation in Caenorhabditis elegans muscle requires a putative F-actin binding site of ADF/cofilin at the C-terminus.Cell motility and the cytoskeleton · 2009Article
- Systematic definition of protein constituents along the major polarization axis reveals an adaptive reuse of the polarization machinery in pheromone-treated budding yeast.Journal of proteome research · 2009Article
- ODA16 aids axonemal outer row dynein assembly through an interaction with the intraflagellar transport machinery.The Journal of cell biology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Actin interacting protein 1 (Aip1p) and cofilin cooperate to disassemble actin filaments in vitro and are thought to promote rapid turnover of actin networks in vivo. The precise method by which Aip1p participates in these activities has not been defined, although severing and barbed-end capping of actin filaments have been proposed. To better describe the mechanisms and biological consequences of Aip1p activities, we undertook an extensive mutagenesis of AIP1 aimed at disrupting and mapping Aip1p interactions. Site-directed mutagenesis suggested that Aip1p has two actin binding sites, the primary actin binding site lies on the edge of its N-terminal beta-propeller and a secondary actin binding site lies in a comparable location on its C-terminal beta-propeller. Random mutagenesis followed by screening for separation of function mutants led to the identification of several mutants specifically defective for interacting with cofilin but still able to interact with actin. These mutants suggested that cofilin binds across the cleft between the two propeller domains, leaving the actin binding sites exposed and flanking the cofilin binding site. Biochemical, genetic, and cell biological analyses confirmed that the actin binding- and cofilin binding-specific mutants are functionally defective, whereas the genetic analyses further suggested a role for Aip1p in an early, internalization step of endocytosis. A complementary, unbiased molecular modeling approach was used to derive putative structures for the Aip1p-cofilin complex, the most stable of which is completely consistent with the mutagenesis data. We theorize that Aip1p-severing activity may involve simultaneous binding to two actin subunits with cofilin wedged between the two actin binding sites of the N- and C-terminal propeller domains.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.