ArticleWorld journal of gastroenterology2005
Expression of matrix metalloproteinases and tissue inhibitor metalloproteinases increases in X-irradiated rat ileum despite the disappearance of CD8a T cells.
Article in World journal of gastroenterology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- An Iatrogenic Model of Brain Small-Vessel Disease: Post-Radiation Encephalopathy.International journal of molecular sciences · 2020Review
- Matrix metalloproteinase expression is altered in the small and large intestine following fractionated radiation in vivo.Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer · 2018Article
- Fractionated abdominal irradiation induces intestinal microvascular changes in an in vivo model of radiotherapy-induced gut toxicity.Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer · 2017Article
- Anti-inflammatory and Anti-apoptotic Effect of Valproic Acid and Doxycycline Independent from MMP Inhibition in Early Radiation Damage.Balkan medical journal · 2016Article
- Role of matrix metalloproteinases in radiation-induced lung injury in alveolar epithelial cells of Bama minipigs.Experimental and therapeutic medicine · 2015Article
- Whole brain radiation-induced cognitive impairment: pathophysiological mechanisms and therapeutic targets.Biomolecules & therapeutics · 2012Review
- Irradiation alters MMP-2/TIMP-2 system and collagen type IV degradation in brain.International journal of radiation oncology, biology, physics · 2012Article
- Gene profiling of the rat medial collateral ligament during early healing using microarray analysis.Journal of applied physiology (Bethesda, Md. : 1985) · 2011Article
- Influence of sublethal total-body irradiation on immune cell populations in the intestinal mucosa.Radiation research · 2010Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo investigate their expression and activity in the rat ileum after exposure to ionizing radiation along with that of the cellular effectors and molecular mediators involved in the regulation of MMPs.
methodsRats were exposed to a single 10-Gy dose of X-rays delivered to the abdomen. A combination of methods, such as zymography, immunohistochemistry and real time reverse transcriptase-polymerase chain reaction, were used to localize and quantify MMPs and the molecules involved in MMP activating and inhibitory pathways (plasmin/plasminogen, TIMPs), CD8+, as well as inflammatory (interleukin (IL)-1beta, IL-8, tumor necrosis factor-alpha, TNF-alpha) and fibrogenic mediators (transforming growth factor-beta1-3) within ileal tissue at 1, 3, and 7 d after irradiation.
resultsA marked increase in MMP-2 and -14 mRNA and protein levels associated with an increased activity of MMP-2 was observed in irradiated ileal tissue. MMP-2 and -14 expression was mainly observed in inflammatory, epithelial, and mesenchymal cells, whereas a slight increase in MMP-3 expression was detected in the few infiltrating macrophages at d 1 after irradiation. Conversely, MMP-1, -7, and -9 mRNA levels were not found to be affected by abdominal irradiation. Irradiation was found to induce disappearance of CD8+ cells. Furthermore, we have observed that TNF-alpha and IL-1beta protein levels increased 6 h after irradiation, whereas those of IL-8 only increased after 3 d and was concomitant with neutrophil infiltration. In addition, the expressions of molecules involved in MMP activating and inhibitory pathways (urokinase-type plasminogen activator and tissue-type plasminogen activator; TIMP-1, TIMP-2, and plasminogen activator-inhibitor-1) were found to be increased after abdominal irradiation.
conclusionThis study showed that abdominal irradiation induces an acute remodeling of the ileum associated with an increased expression of MMPs and TIMPs that do not involve CD8+ T cells but involve mesenchymal and epithelial cells, although to a lesser extent, and probably even soluble inflammatory and fibrogenic mediators.
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