ReviewClinical microbiology reviews2006
Real-time PCR in clinical microbiology: applications for routine laboratory testing.
Review in Clinical microbiology reviews, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 423 papers, 3 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
423 citing papers in PubMed, 3 syntheses or guidelines pooled it, 1,317 citations in OpenAlex.
- Diagnostic accuracy of genetic markers and nucleic acid techniques for the detection of Leptospira in clinical samples: A meta-analysis.PLoS neglected tropical diseases · 2020Pooled it
- Diagnostic accuracy of in-house real-time PCR assay for Mycobacterium tuberculosis: a systematic review and meta-analysis.BMC infectious diseases · 2019Pooled it
- Effectiveness of real-time polymerase chain reaction assay for the detection of Mycobacterium tuberculosis in pathological samples: a systematic review and meta-analysis.Systematic reviews · 2017Pooled it
- Molecular pathogenesis, global epidemiological trends, and treatment strategies for pteropine orthoreoviruses: a narrative review.Emerging microbes & infections · 2026Review
- Research Progress in Applications of the CRISPR-Cas12b System in Pathogen Nucleic Acid Detection.Current issues in molecular biology · 2026Review
- Development and Laboratory Evaluation of aInternational journal of molecular sciences · 2026Article
- Dielectrophoretic Enrichment Coupled with Impedance Spectroscopy for Real-Time Bacterial Detection and Antibiotic Susceptibility Testing Using an Interdigitated Wave Electrode Array.Sensors (Basel, Switzerland) · 2026Article
- Quantitative real-time PCR in the diagnosis of opportunistic infections in immunocompromised patients: Diagnostic performance, clinical utility, and future directions.Infectious diseases & immunity · 2026Review
- Diagnostics for priority bacterial pathogens: global gaps and research needs for curbing antimicrobial resistance in low-resource settings.The Lancet. Microbe · 2026Review
- Development and validation of a crystal digital PCR assay for duck Tembusu virus detection and vaccine monitoring.Poultry science · 2026Article
- Article
- Comparative Evaluation of Multiplex Real-Time PCR, Standard Urine Culture, and Rapid Nephelometric Screening in Patients with Complicated Urinary Tract Infections.Diagnostics (Basel, Switzerland) · 2026Article
- Tuberculosis Diagnostic Methods: Clinical Applicability, Implementation Challenges, and Integrated Testing Strategies.Pathogens (Basel, Switzerland) · 2026Review
- High-Resolution Melting Curve Analysis (HRMA) for the Identification of Class D β-Lactamases (CHDLs) inInfection and drug resistance · 2026Article
- First Isolation of Bovine Coronavirus From Yanbian, China, and Analytical Validation of a SYBR Green I RT-qPCR Panel for Calf Diarrhea Viruses.Transboundary and emerging diseases · 2026Article
- Performance evaluation of a novel fully-automated molecular diagnostics system Molecision R8.PloS one · 2026Article
- Advances in biosensors for bacterial detection and identification.World journal of microbiology & biotechnology · 2025Review
- DNA-directed electrochemiluminescence nanosphere with electrocatalysis-enhanced microfluidic arrays for rapid multibacterial detection.Science advances · 2025Article
- Identification of enterovirus 71 hand, foot and mouth disease from other enteroviruses by microfluidic digital PCR.BMC infectious diseases · 2025Article
- Rapid Visual Detection of Senecavirus A Based on RPA-CRISPR/Cas12a System with Canonical or Suboptimal PAM.Viruses · 2025Article
363 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Real-time PCR has revolutionized the way clinical microbiology laboratories diagnose many human microbial infections. This testing method combines PCR chemistry with fluorescent probe detection of amplified product in the same reaction vessel. In general, both PCR and amplified product detection are completed in an hour or less, which is considerably faster than conventional PCR detection methods. Real-time PCR assays provide sensitivity and specificity equivalent to that of conventional PCR combined with Southern blot analysis, and since amplification and detection steps are performed in the same closed vessel, the risk of releasing amplified nucleic acids into the environment is negligible. The combination of excellent sensitivity and specificity, low contamination risk, and speed has made real-time PCR technology an appealing alternative to culture- or immunoassay-based testing methods for diagnosing many infectious diseases. This review focuses on the application of real-time PCR in the clinical microbiology laboratory.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.