ArticleGenes & development2006
Tissue-specific RNAi reveals that WT1 expression in nurse cells controls germ cell survival and spermatogenesis.
Article in Genes & development, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
51 citing papers in PubMed, 116 citations in OpenAlex.
- The application of experimental models for the drug discovery for digestive tumors.Molecular cancer · 2026Review
- A Study ofGenes · 2024Article
- Characterization of Two Gonadal Genes, zar1 and wt1b, in Hermaphroditic Fish Asian Seabass (Animals : an open access journal from MDPI · 2024Article
- Rhox8 homeobox gene ablation leads to rete testis abnormality and male subfertility in mice†.Biology of reproduction · 2023Article
- Expression of Protein Markers in Spermatogenic and Supporting Sertoli Cells Affected by High Abdominal Temperature in Cryptorchidism Model Mice.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2023Article
- Cisplatin Induces Apoptosis in Mouse Neonatal Testes Organ Culture.International journal of molecular sciences · 2022Article
- Immature rat testis sustained long-term development using an integrative model.Biological research · 2022Article
- Small RNA perspective of physical exercise-related improvement of male reproductive dysfunction due to obesity.Frontiers in endocrinology · 2022Article
- Mediterranean Pine Vole,Animals : an open access journal from MDPI · 2021Article
- Gene and histomorphology alteration analysis in spermatogenesis arrest mouse model: a probable novel approach for infertility.Central European journal of urology · 2021Article
- WT1 expression in vessels varies with histopathological grade in tumour-bearing and control tissue from patients with breast cancer.British journal of cancer · 2018Article
- Cross-talk between miR-471-5p and autophagy component proteins regulates LC3-associated phagocytosis (LAP) of apoptotic germ cells.Nature communications · 2017Article
- Rhox8 Ablation in the Sertoli Cells Using a Tissue-Specific RNAi Approach Results in Impaired Male Fertility in Mice.Biology of reproduction · 2015Article
- UPF2, a nonsense-mediated mRNA decay factor, is required for prepubertal Sertoli cell development and male fertility by ensuring fidelity of the transcriptome.Development (Cambridge, England) · 2015Article
- Wilms' tumor suppressor gene (WT1) suppresses apoptosis by transcriptionally downregulating BAX expression in immature rat granulosa cells.Journal of ovarian research · 2014Article
- Mechanisms of transcriptional regulation by WT1 (Wilms' tumour 1).The Biochemical journal · 2014Review
- Effects of WT1 gene downregulation on apoptosis in porcine fetal fibroblasts.In vitro cellular & developmental biology. Animal · 2014Article
- Accelerating cancer modeling with RNAi and nongermline genetically engineered mouse models.Cold Spring Harbor protocols · 2013Review
- Small interfering RNA-mediated translation repression alters ribosome sensitivity to inhibition by cycloheximide in Chlamydomonas reinhardtii.The Plant cell · 2013Article
- The Wilms tumor gene, Wt1, is critical for mouse spermatogenesis via regulation of sertoli cell polarity and is associated with non-obstructive azoospermia in humans.PLoS genetics · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using a novel tissue-specific RNA interference (RNAi) approach that mimics the principle by which naturally occurring microRNAs (miRNA) are made, we demonstrate that the Wilms' tumor 1 (WT1) transcription factor has an essential role in spermatogenesis. Mice depleted of WT1 in Sertoli nurse cells suffered from increased germ cell apoptosis, loss of adherens junctions, disregulation of adherence junction-associated genes, and impaired fertility. These effects were recapitulated in transgenic mice expressing a dominant-negative form of WT1 in Sertoli cells, demonstrating the validity of our RNAi approach. Our results indicate that the tumor suppressor WT1 promotes Sertoli cell-germ cell signaling events driving spermatogenesis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.