ArticleProceedings of the National Academy of Sciences of the United States of America2006
Detachment of affinity-captured bioparticles by elastic deformation of a macroporous hydrogel.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Mechanical and Light Activation of Materials for Chemical Production.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Article
- Past, Present, and Future of Affinity-based Cell Separation Technologies.Acta biomaterialia · 2020Review
- Efficient release of immunocaptured cells using coiled-coils in a microfluidic device.RSC advances · 2019Article
- Preparation of Gelatin and Gelatin/Hyaluronic Acid Cryogel Scaffolds for the 3D Culture of Mesothelial Cells and Mesothelium Tissue Regeneration.International journal of molecular sciences · 2019Article
- Strategies to Maximize the Potential of Marine Biomaterials as a Platform for Cell Therapy.Marine drugs · 2016Review
- Disposable polymeric cryogel bioreactor matrix for therapeutic protein production.Nature protocols · 2013Article
- Monosaccharide-responsive phenylboronate-polyol cell scaffolds for cell sheet and tissue engineering applications.PloS one · 2013Article
- Three-dimensional supermacroporous carrageenan-gelatin cryogel matrix for tissue engineering applications.BioMed research international · 2013Article
- Tag-free microfluidic separation of cells against multiple markers.Analytical chemistry · 2012Article
- Active scaffolds for on-demand drug and cell delivery.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Thermoresponsive poly(N-vinylcaprolactam) cryogels: synthesis and its biophysical evaluation for tissue engineering applications.Journal of materials science. Materials in medicine · 2010Article
- Proliferation of myoblast skeletal cells on three-dimensional supermacroporous cryogels.International journal of biological sciences · 2010Article
- Macroporous interpenetrating cryogel network of poly(acrylonitrile) and gelatin for biomedical applications.Journal of materials science. Materials in medicine · 2009Article
- Chromato-panning: an efficient new mode of identifying suitable ligands from phage display libraries.BMC biotechnology · 2009Article
Corrections and comments
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Authors and funding
4 authors.
Funding
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Abstract
Adsorption of bioparticles to affinity surfaces involves polyvalent interactions, complicating greatly the recovery of the adsorbed material. A unique system for the efficient binding and release of different cells and particles is described. Affinity-bound bioparticles and synthetic particles are detached from the macroporous hydrogel matrix, a so-called cryogel, when the cryogel undergoes elastic deformation. The particle detachment upon elastic deformation is believed to be due to breaking of many of the multipoint attachments between the particles and the affinity matrix and the change in the distance between affinity ligands when the matrix is deformed. However, no release of affinity-bound protein occurred upon elastic deformation. The phenomenon of particle detachment upon elastic deformation is believed to be of a generic nature, because it was demonstrated for a variety of bioparticles of different sizes and for synthetic particles, for different ligand-receptor pairs (IgG-protein A, sugar-ConA, metal ion-chelating ligand), and when the deformation was caused by either external forces (mechanical deformation) or internal forces (the shrinkage of thermosensitive, macroporous hydrogel upon an increase in temperature). The elasticity of cryogel monoliths ensures high recovery of captured cells under mild conditions, with highly retained viability. This property, along with their continuous porous structure makes cryogel monoliths very attractive for applications in affinity cell separation.
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