ArticleBiophysical journal2006
Micro- and macrorheological properties of actin networks effectively cross-linked by depletion forces.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Morphological control of bundled actin networks subject to fixed-mass depletion.The Journal of chemical physics · 2024Article
- Highly flexible PEG-LifeAct constructs act as tunable biomimetic actin crosslinkers.Soft matter · 2024Article
- Water-Driven Sol-Gel Transition in Native Cellulose/1-Ethyl-3-methylimidazolium Acetate Solutions.ACS macro letters · 2024Article
- Crowding alters F-actin secondary structure and hydration.Communications biology · 2023Article
- Heavy water induces bundling in entangled actin networks.RSC advances · 2023Article
- Micro-mechanical response and power-law exponents from the longitudinal fluctuations of F-actin solutions.Soft matter · 2023Article
- Regulation of Actin Bundle Mechanics and Structure by Intracellular Environmental Factors.Frontiers in physics · 2021Article
- Crowding tunes the organization and mechanics of actin bundles formed by crosslinking proteins.FEBS letters · 2021Article
- Actin Bundle Nanomechanics and Organization Are Modulated by Macromolecular Crowding and Electrostatic Interactions.Frontiers in molecular biosciences · 2021Article
- Polarisome scaffolder Spa2-mediated macromolecular condensation of Aip5 for actin polymerization.Nature communications · 2019Article
- Organization of associating or crosslinked actin filaments in confinement.Cytoskeleton (Hoboken, N.J.) · 2019Article
- Architectural Dynamics of CaMKII-Actin Networks.Biophysical journal · 2019Article
- Multiscale mechanobiology: computational models for integrating molecules to multicellular systems.Integrative biology : quantitative biosciences from nano to macro · 2015Review
- Nonlinear viscoelasticity of actin transiently cross-linked with mutant α-actinin-4.Journal of molecular biology · 2011Article
- Elasticity in ionically cross-linked neurofilament networks.Biophysical journal · 2010Article
- Active biological materials.Annual review of physical chemistry · 2009Review
- Rheological characterization of the bundling transition in F-actin solutions induced by methylcellulose.PloS one · 2008Article
- Helical twist controls the thickness of F-actin bundles.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Micro- and macrorheological properties of isotropically cross-linked actin networks.Biophysical journal · 2008Article
- Mechanical and structural properties of in vitro neurofilament hydrogels.European biophysics journal : EBJ · 2007Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The structure and rheology of cytoskeletal networks are regulated by actin binding proteins. Aside from these specific interactions, depletion forces can also alter the properties of cytoskeletal networks. Here we demonstrate that the addition of poly(ethylene glycol) (PEG) as a depletion agent results not only in severe structural changes, but also in alterations in mechanical properties of actin solutions. In the plateau of the elastic modulus two regimes can be distinguished by micro and macrorheological methods. In the first, the elastic modulus increases only slightly with increasing depletion agent, whereas above a critical concentration c*, a strong increase of cPEG6k3.5 is observed in a distinct second regime. Microrheological data and electron microscopy images show a homogenous network of actin filaments in the first regime, whereas at higher PEG concentrations a network of actin bundles is observed. The concentration dependence of the plateau modulus G0, the shift in entanglement time taue, and the nonlinear response indicate that below c* the network becomes effectively cross-linked, whereas above c* G0(cPEG6k) is primarily determined by the network of bundles that exhibits a linearly increasing bundle thickness.
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