ArticleBiophysical journal2006
Revealing the topography of cellular membrane domains by combined atomic force microscopy/fluorescence imaging.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 76 citations in OpenAlex.
- Spatial Deconstruction of the Plasma Membrane.Journal of the American Chemical Society · 2026Article
- Cyclodextrins: Only Pharmaceutical Excipients or Full-Fledged Drug Candidates?Pharmaceutics · 2022Review
- Cell membrane sample preparation method of combined AFM and dSTORM analysis.Biophysics reports · 2022Article
- Recent progress of surface-enhanced Raman spectroscopy for subcellular compartment analysis.Theranostics · 2021Review
- Receptor-mediated endocytosis generates nanomechanical force reflective of ligand identity and cellular property.Journal of cellular physiology · 2018Article
- Tissue Force Programs Cell Fate and Tumor Aggression.Cancer discovery · 2017Review
- Progress in the Correlative Atomic Force Microscopy and Optical Microscopy.Sensors (Basel, Switzerland) · 2017Review
- Multi-protein assemblies underlie the mesoscale organization of the plasma membrane.Nature communications · 2014Article
- Observational
- Regulation from within: the cytoskeleton in transmembrane signaling.Trends in cell biology · 2012Review
- Particulate allergens potentiate allergic asthma in mice through sustained IgE-mediated mast cell activation.The Journal of clinical investigation · 2011Article
- Spatio-temporal signaling in mast cells.Advances in experimental medicine and biology · 2011Review
- Applications of atomic force microscopy in biophysical chemistry of cells.The journal of physical chemistry. B · 2010Article
- Liver X Receptor activation downregulates AKT survival signaling in lipid rafts and induces apoptosis of prostate cancer cells.Oncogene · 2010Article
- Probing membrane order and topography in supported lipid bilayers by combined polarized total internal reflection fluorescence-atomic force microscopy.Biophysical journal · 2009Article
- Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Impact of actin rearrangement and degranulation on the membrane structure of primary mast cells: a combined atomic force and laser scanning confocal microscopy investigation.Biophysical journal · 2009Article
- Cross-linking of FcepsilonRI causes Ca2+ mobilization via a sphingosine kinase pathway in a clathrin-dependent manner.Cell calcium · 2009Article
- High-resolution three-dimensional imaging of the rich membrane structures of bone marrow-derived mast cells.Ultramicroscopy · 2008Article
- In vitro activities of ER-119884 and E5700, two potent squalene synthase inhibitors, against Leishmania amazonensis: antiproliferative, biochemical, and ultrastructural effects.Antimicrobial agents and chemotherapy · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
Simultaneous atomic force microscopy (AFM) and confocal fluorescence imaging were used to observe in aqueous buffer the three-dimensional landscape of the inner surface of membrane sheets stripped from fixed tumor mast cells. The AFM images reveal prominent, irregularly shaped raised domains that label with fluorescent markers for both resting and activated immunoglobin E receptors (FcepsilonRI), as well as with cholera toxin-aggregated GM1 and clathrin. The latter suggests that coated pits bud from these regions. These features are interspersed with flatter regions of membrane and are frequently surrounded and interconnected by cytoskeletal assemblies. The raised domains shrink in height by approximately 50% when cholesterol is extracted with methyl-beta-cyclodextrin. Based on composition, the raised domains seen by AFM correspond to the cholesterol-enriched dark patches observed in transmission electron microscopy (TEM). These patches were previously identified as sites of signaling and endocytosis based on their localization of activated FcepsilonRI, at least 10 associated signaling molecules, and the presence of clathrin-coated pits. Overall the data suggest that signaling and endocytosis occur in mast cells from raised membrane regions that depend on cholesterol for their integrity and may be organized in specific relationship with the cortical cytoskeleton.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.