ArticleJournal of virology2006
Stable growth of wild-type hepatitis A virus in cell culture.
Article in Journal of virology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 63 citations in OpenAlex.
- The hepatocyte traffic network in the human hepatitis A virus biological cycle from an evolutionary perspective.Communications biology · 2025Article
- Review
- Lessons from intensified surveillance of viral hepatitis A, Israel, 2017 and 2018.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2021Article
- Exosome mimicry by a HAVCR1-NPC1 pathway of endosomal fusion mediates hepatitis A virus infection.Nature microbiology · 2020Article
- Discovery of Components Acting as the Obstacles in the Detection of Enteric Viruses from Berries.Food and environmental virology · 2020Article
- Review
- Novel opportunities for NGS-based one health surveillance of foodborne viruses.One health outlook · 2020Review
- Laser-induced fluorescence (LIF) as a smart method for fast environmental virological analyses: validation on Picornaviruses.Scientific reports · 2019Article
- Human Astrovirus MLB ReplicationJournal of virology · 2019Article
- HAVCR1 (CD365) and Its Mouse Ortholog Are Functional Hepatitis A Virus (HAV) Cellular Receptors That Mediate HAV Infection.Journal of virology · 2018Article
- Determinants in the Ig Variable Domain of Human HAVCR1 (TIM-1) Are Required To Enhance Hepatitis C Virus Entry.Journal of virology · 2018Article
- Cellular cap-binding protein, eIF4E, promotes picornavirus genome restructuring and translation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- New Methods in Tissue Engineering: Improved Models for Viral Infection.Annual review of virology · 2014Article
- Nonlytic viral spread enhanced by autophagy components.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Molecular biology and inhibitors of hepatitis A virus.Medicinal research reviews · 2014Review
- Inhibition of cellular autophagy deranges dengue virion maturation.Journal of virology · 2013Article
- Disruption of TLR3 signaling due to cleavage of TRIF by the hepatitis A virus protease-polymerase processing intermediate, 3CD.PLoS pathogens · 2011Article
- A polymorphism in TIM1 is associated with susceptibility to severe hepatitis A virus infection in humans.The Journal of clinical investigation · 2011Article
- Nucleotide variability and translation efficiency of the 5' untranslated region of hepatitis A virus: update from clinical isolates associated with mild and severe hepatitis.Journal of virology · 2010Article
- Determinants in 3Dpol modulate the rate of growth of hepatitis A virus.Journal of virology · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human wild-type (wt) hepatitis A virus (HAV), the causative agent of acute hepatitis, barely grows in cell culture and in the process accumulates attenuating and cell culture-adapting mutations. This genetic instability of wt HAV in cell culture is a major roadblock to studying HAV pathogenesis and producing live vaccines that are not overly attenuated for humans. To develop a robust cell culture system capable of supporting the efficient growth of wt HAV, we transfected different cell lines with in vitro RNA transcripts of wt HAV containing the blasticidin resistance gene. Blasticidin-resistant colonies grew only in transfected Huh7 cells and produced infectious virus. HAV was genetically stable in Huh7 cells for at least nine serial passages and did not accumulate attenuating or cell culture-adapting mutations. Treatment with alpha interferon A/D cured the blasticidin-resistant Huh7 cells of the HAV infection. The cured cells, termed Huh7-A-I cells, did not contain virus or HAV antigens and were sensitive to blasticidin. Huh7-A-I cells were more permissive than parental cells for wt HAV infection, including a natural isolate from a human stool sample, and produced 10-fold-more infectious particles. This is the first report of a cell line that allows the genetically stable growth of human wt HAV. The viral vectors and cells described here should allow better insight into the pathogenesis of HAV and the development of attenuated vaccines. The cell lines susceptible to wt HAV growth may also be used to detect and isolate infectious virus from patient and environmental samples.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.