ArticleNucleic acids research1992
The Saccharomyces cerevisiae MGT1 DNA repair methyltransferase gene: its promoter and entire coding sequence, regulation and in vivo biological functions.
Article in Nucleic acids research, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 42 citations in OpenAlex.
- Review
- Requirement of the Saccharomyces cerevisiae APN1 gene for the repair of mitochondrial DNA alkylation damage.Environmental and molecular mutagenesis · 2009Article
- Two proteolytic pathways regulate DNA repair by cotargeting the Mgt1 alkylguanine transferase.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- O6-methylguanine-DNA methyltransferase deficiency in developing brain: implications for brain tumorigenesis.DNA repair · 2007Article
- Direct reversal of DNA alkylation damage.Chemical reviews · 2006Review
- How heterologously expressed Escherichia coli genes contribute to understanding DNA repair processes in Saccharomyces cerevisiae.Current genetics · 2004Review
- Influence of S-adenosylmethionine pool size on spontaneous mutation, dam methylation, and cell growth of Escherichia coli.Journal of bacteriology · 1999Article
- Thermostable archaeal O6-alkylguanine-DNA alkyltransferases.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- The Pol beta-14 dominant negative rat DNA polymerase beta mutator mutant commits errors during the gap-filling step of base excision repair in Saccharomyces cerevisiae.Journal of bacteriology · 1998Article
- Identification and characterization of Saccharomyces cerevisiae EXO1, a gene encoding an exonuclease that interacts with MSH2.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- 3-methyladenine-DNA-glycosylase and O6-alkyl guanine-DNA-alkyltransferase activities and sensitivity to alkylating agents in human cancer cell lines.British journal of cancer · 1996Article
- Promoter elements of the PHR1 gene of Saccharomyces cerevisiae and their roles in the response to DNA damage.Nucleic acids research · 1995Article
- Replication protein A binds to regulatory elements in yeast DNA repair and DNA metabolism genes.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- The Escherichia coli AlkB protein protects human cells against alkylation-induced toxicity.Journal of bacteriology · 1994Article
- A common element involved in transcriptional regulation of two DNA alkylation repair genes (MAG and MGT1) of Saccharomyces cerevisiae.Molecular and cellular biology · 1993Article
- In vivo evidence for endogenous DNA alkylation damage as a source of spontaneous mutation in eukaryotic cells.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- New nucleotide sequence data on the EMBL File Server.Nucleic acids research · 1992Article
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
We previously cloned a yeast DNA fragment that, when fused with the bacterial lacZ promoter, produced O6-methylguanine DNA repair methyltransferase (MGT1) activity and alkylation resistance in Escherichia coli (Xiao et al., EMBO J. 10,2179). Here we describe the isolation of the entire MGT1 gene and its promoter by sequence directed chromosome integration and walking. The MGT1 promoter was fused to a lacZ reporter gene to study how MGT1 expression is controlled. MGT1 is not induced by alkylating agents, nor is it induced by other DNA damaging agents such as UV light. However, deletion analysis defined an upstream repression sequence, whose removal dramatically increased basal level gene expression. The polypeptide deduced from the complete MGT1 sequence contained 18 more N-terminal amino acids than that previously determined; the role of these 18 amino acids, which harbored a potential nuclear localization signal, was explored. The MGT1 gene was also cloned under the GAL1 promoter, so that MTase levels could be manipulated, and we examined MGT1 function in a MTase deficient yeast strain (mgt1). The extent of resistance to both alkylation-induced mutation and cell killing directly correlated with MTase levels. Finally we show that mgt1 S.cerevisiae has a higher rate of spontaneous mutation than wild type cells, indicating that there is an endogenous source of DNA alkylation damage in these eukaryotic cells and that one of the in vivo roles of MGT1 is to limit spontaneous mutations.
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