ArticleBMC developmental biology2006
Proteomics of early zebrafish embryos.
Article in BMC developmental biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 163 papers.
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Who cites it
163 citing papers in PubMed.
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- In vivo screen reveals specific roles of Hippo pathway components in development and regeneration.Life science alliance · 2026Article
- SureCLIP and DrCLIP: Genetic manipulation-free, generalizable interactome mapping for cells and zebrafish.bioRxiv : the preprint server for biology · 2025Article
- Dynamic expression of the zebrafish (Danio rerio) proteome across early larval development.Comparative biochemistry and physiology. Part D, Genomics & proteomics · 2025Article
- Stable Isotope Labeling in Bacteria Enables Characterization and Quantification of Frataxin Protein in a Friedreich's Ataxia Zebrafish Model.Analytical chemistry · 2025Article
- Zebrafish and cellular models of SELENON-Congenital myopathy exhibit novel embryonic and metabolic phenotypes.Skeletal muscle · 2025Article
- Increasingly efficient chromatin binding of cohesin and CTCF supports chromatin architecture formation during zebrafish embryogenesis.Nature communications · 2025Article
- Article
- The dynamics and functional impact of tRNA repertoires during early embryogenesis in zebrafish.The EMBO journal · 2024Article
- Protein profiling of zebrafish embryos unmasks regulatory layers during early embryogenesis.Cell reports · 2024Article
- A dual ribosomal system in the zebrafish soma and germline.bioRxiv : the preprint server for biology · 2024Article
- A novel DSP zebrafish model reveals training- and drug-induced modulation of arrhythmogenic cardiomyopathy phenotypes.Cell death discovery · 2023Article
- Antioxidant, Pro-Survival and Pro-Regenerative Effects of Conditioned Medium from Wharton's Jelly Mesenchymal Stem Cells on Developing Zebrafish Embryos.International journal of molecular sciences · 2023Article
- Chromatin expansion microscopy reveals nanoscale organization of transcription and chromatin.Science (New York, N.Y.) · 2023Article
- Triphenyl phosphate-induced pericardial edema in zebrafish embryos is dependent on the ionic strength of exposure media.Environment international · 2023Article
- Maternal mRNA deadenylation and allocation via Rbm14 condensates facilitate vertebrate blastula development.The EMBO journal · 2023Article
- Mob4 is required for neurodevelopment in zebrafish.microPublication biology · 2023Article
- Zebrafish: A Tractable Model for Analysis of T Cell Development.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Assessment of endocytic traffic and Ocrl function in the developing zebrafish neuroepithelium.Journal of cell science · 2022Article
103 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundZebrafish (D. rerio) has become a powerful and widely used model system for the analysis of vertebrate embryogenesis and organ development. While genetic methods are readily available in zebrafish, protocols for two dimensional (2D) gel electrophoresis and proteomics have yet to be developed.
resultsAs a prerequisite to carry out proteomic experiments with early zebrafish embryos, we developed a method to efficiently remove the yolk from large batches of embryos. This method enabled high resolution 2D gel electrophoresis and improved Western blotting considerably. Here, we provide detailed protocols for proteomics in zebrafish from sample preparation to mass spectrometry (MS), including a comparison of databases for MS identification of zebrafish proteins.
conclusionThe provided protocols for proteomic analysis of early embryos enable research to be taken in novel directions in embryogenesis.
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