ArticleCell death and differentiation2006
Bioenergetic analysis of cerebellar granule neurons undergoing apoptosis by potassium/serum deprivation.
Article in Cell death and differentiation, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Exploration of the Neuromodulatory Properties of Fyn and GSK-3β Kinases Exploiting 7-Azaindole-Based Inhibitors.Journal of medicinal chemistry · 2025Article
- Differential ROS-Mediated Phosphorylation of Drp1 in Mitochondrial Fragmentation Induced by Distinct Cell Death Conditions in Cerebellar Granule Neurons.Oxidative medicine and cellular longevity · 2021Article
- Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons.Journal of visualized experiments : JoVE · 2017Article
- Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture.Journal of visualized experiments : JoVE · 2017Article
- (13)C metabolic flux analysis in neurons utilizing a model that accounts for hexose phosphate recycling within the pentose phosphate pathway.Neurochemistry international · 2016Article
- High-throughput screening of FDA-approved drugs using oxygen biosensor plates reveals secondary mitofunctional effects.Mitochondrion · 2014Article
- Models of calcium dynamics in cerebellar granule cells.Cerebellum (London, England) · 2012Review
- Adaptation of microplate-based respirometry for hippocampal slices and analysis of respiratory capacity.Journal of neuroscience research · 2011Article
- Assessing mitochondrial dysfunction in cells.The Biochemical journal · 2011Review
- Redox regulation of the intrinsic pathway in neuronal apoptosis.Antioxidants & redox signaling · 2011Review
- The marine sponge metabolite mycothiazole: a novel prototype mitochondrial complex I inhibitor.Bioorganic & medicinal chemistry · 2010Article
- The sites and topology of mitochondrial superoxide production.Experimental gerontology · 2010Review
- Simultaneous single neuron recording of O2 consumption, [Ca2+]i and mitochondrial membrane potential in glutamate toxicity.Journal of neurochemistry · 2009Article
- A systems biology approach to investigating apoptotic stimuli as effectors of cell metabolism: practical application of top-down control analysis to attached neurons.International journal of molecular sciences · 2009Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Apoptosis induced by K+/serum deprivation (low K+) in cerebellar granule neurons has been extensively investigated. The mitochondria play a key role in apoptosis by releasing proapoptotic factors into the cytoplasm, and mitochondrial dysfunction has been proposed as an early or initiating event in this model. To directly test this hypothesis, cellular and mitochondrial bioenergetics were quantified by determining the respiratory parameters of coverslip-attached neurons. While oxidative phosphorylation rate decreased 39-49% in low K+, this was due to decreased cellular ATP demand rather than impaired ATP/ADP exchange or respiratory chain inhibition. From 3 to 5 h in low K+, apoptosis progressed from 13 to 40% despite no appreciable change in respiratory parameters. Changes in steady-state O2-, assessed with dihydroethidium, were seen in granule but not hippocampal neurons. The O2- change correlated with changes in [Ca2+]c, but not mitochondrial respiration. Thus, early mitochondrial dysfunction can be excluded in this common model of neuronal apoptosis.
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Registered trials
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