ArticleFolia microbiologica2005
Visualization of DNA-containing structures in various species of Chlorophyta, Rhodophyta and Cyanophyta using SYBR Green I dye.
Article in Folia microbiologica, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Growth and Cell Size of MicroalgaMicroorganisms · 2024Article
- Optimized Protocol for Microalgae DNA Staining with SYTO9/SYBR Green I, Based on Flow Cytometry and RSM Methodology: Experimental Design, Impacts and Validation.Methods and protocols · 2022Article
- In Silico and Cellular Differences Related to the Cell Division Process between the A and B Races of the Colonial MicroalgaBiomolecules · 2021Article
- Toxicity testing of indocyanine green and fluorodeoxyglucose conjugated iron oxide nanoparticles with and without exposure to a magnetic field.Asian journal of nanoscience and materials · 2021Article
- Article
- Characterization of Growth and Cell Cycle Events Affected by Light Intensity in the Green AlgaBiomolecules · 2021Article
- Article
- Routine Management of Microalgae Using Autofluorescence from Chlorophyll.Molecules (Basel, Switzerland) · 2019Review
- Applicability of Automated Cell Counter with a Chlorophyll Detector in Routine Management of Microalgae.Scientific reports · 2018Article
- Deciphering the relationship among phosphate dynamics, electron-dense body and lipid accumulation in the green alga Parachlorella kessleri.Scientific reports · 2016Article
- DNA damage during G2 phase does not affect cell cycle progression of the green alga Scenedesmus quadricauda.PloS one · 2011Article
- Zygnematalean zygospores: morphological features and use in species identification.Folia microbiologica · 2007Article
- Development of alternative support system for viable count of cyanobacteria by most probable number method.Folia microbiologica · 2006Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed an alternative method of staining cell nuclei and chloroplast nucleoids of algal cells using SYBR Green I (the fluorescent dye used commonly for detecting dsDNA in agarose and polyacrylamide gels as an alternative to highly mutagenic ethidium bromide and for DNA staining of viruses and bacteria followed by flow cytometry, digital image analysis or real-time PCR), which enabled routine staining in vivo. Cells do not need to be fixed or treated chemically or physically before staining, thus the shape, size and position of DNA-containing structures are not affected. The fluorescence signal is sharp and reproducible. Examples of application of the method are shown in color microphotographs for representatives of eukaryotic algae from the taxa Chlorophyta, Rhodophyta and the prokaryotic Cyanophyta. The method is also useful for studying progress of the cell cycle in algal cells dividing by multiple fission, as shown by observation of changes in nuclear number during the cell cycle of the green alga Chlamydomonas reinhardtii and Scenedesmus quadricauda. Staining with SYBR Green I can be recommended as a fast, safe and efficient method for the detection of DNA-containing structures in vivo.
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