ArticleNature chemical biology2005
Preferential synapsis of loxP sites drives ordered strand exchange in Cre-loxP site-specific recombination.
Article in Nature chemical biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- Clusterin elaborated by renal tubular epithelial cells under high oxalate stress serves as a matrix protein to facilitate kidney stone formation.Cellular and molecular life sciences : CMLS · 2026Article
- Minimal mutational requirements for conversion of a telomere resolvase into a Cre-like site-specific recombinase.PloS one · 2026Article
- Article
- Dynamics in Cre-loxP site-specific recombination.Current opinion in structural biology · 2024Review
- Orthogonal LoxPsym sites allow multiplexed site-specific recombination in prokaryotic and eukaryotic hosts.Nature communications · 2024Article
- Design and characterization of hyperactive mutants of the Agrobacterium tumefaciens telomere resolvase, TelA.PloS one · 2024Article
- Mechanisms of Cre recombinase synaptic complex assembly and activation illuminated by Cryo-EM.Nucleic acids research · 2022Article
- DNA binding induces aProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Synthesis of 5'-Thio-3'-O-ribonucleoside Phosphoramidites.The Journal of organic chemistry · 2017Article
- Site-specific bacterial chromosome engineering mediated by IntA integrase from Rhizobium etli.BMC microbiology · 2016Article
- Single molecule TPM analysis of the catalytic pentad mutants of Cre and Flp site-specific recombinases: contributions of the pentad residues to the pre-chemical steps of recombination.Nucleic acids research · 2015Article
- Insights into the preferential order of strand exchange in the Cre/loxP recombinase system: impact of the DNA spacer flanking sequence and flexibility.Journal of computer-aided molecular design · 2015Article
- TPM analyses reveal that FtsK contributes both to the assembly and the activation of the XerCD-dif recombination synapse.Nucleic acids research · 2014Article
- Crystal structure of an intermediate of rotating dimers within the synaptic tetramer of the G-segment invertase.Nucleic acids research · 2013Article
- Capturing reaction paths and intermediates in Cre-loxP recombination using single-molecule fluorescence.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Single-molecule microscopy of Cre recombination.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Sin resolvase catalytic activity and oligomerization state are tightly coupled.Journal of molecular biology · 2010Article
- Restoration of catalytic functions in Cre recombinase mutants by electrostatic compensation between active site and DNA substrate.Nucleic acids research · 2010Article
- Requirements for catalysis in the Cre recombinase active site.Nucleic acids research · 2010Article
- Control of directionality in the DNA strand-exchange reaction catalysed by the tyrosine recombinase TnpI.Nucleic acids research · 2010Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bacteriophage P1 Cre recombinase catalyzes site-specific recombination between 34-base-pair loxP sequences in a variety of topological contexts. This reaction is widely used to manipulate DNA molecules in applications ranging from benchtop cloning to genome modifications in transgenic animals. Despite the simple, highly symmetric nature of the Cre-loxP system, there is strong evidence that the reaction is asymmetric; the 'bottom' strands in the recombining loxP sites are preferentially exchanged before the 'top' strands. Here, we address the mechanistic basis for ordered strand exchange in the Cre-loxP recombination pathway. Using suicide substrates containing 5'-bridging phosphorothioate linkages at both cleavage sites, fluorescence resonance energy transfer between synapsed loxP sites and a Cre mutant that can cleave the bridging phosphorothioate linkage but not a normal phosphodiester linkage, we showed that preferential formation of a specific synaptic complex between loxP sites imposes ordered strand exchange during recombination and that synapsis stimulates cleavage of loxP sites.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.