ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2006
Peptidomics of a single identified neuron reveals diversity of multiple neuropeptides with convergent actions on cellular excitability.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Feeding state-specific hormonal tuning of neural circuit modulation.Journal of neurophysiology · 2025Article
- Origins, Technological Advancement, and Applications of Peptidomics.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Peptidomics.Nature reviews. Methods primers · 2023Article
- Ion channel profiling of the Lymnaea stagnalis ganglia via transcriptome analysis.BMC genomics · 2021Article
- Neuropeptide Localization inFrontiers in molecular neuroscience · 2021Review
- Early Metazoan Origin and Multiple Losses of a Novel Clade of RIM Presynaptic Calcium Channel Scaffolding Protein Homologs.Genome biology and evolution · 2020Article
- Strategies for the Identification of Bioactive Neuropeptides in Vertebrates.Frontiers in neuroscience · 2019Review
- Exploring the Sea Urchin Neuropeptide Landscape by Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2018Article
- Phosphopeptidomics Reveals Differential Phosphorylation States and Novel SxE Phosphosite Motifs of Neuropeptides in Dense Core Secretory Vesicles.Journal of the American Society for Mass Spectrometry · 2018Article
- Neuroproteomics: How Many Angels can be Identified in an Extract from the Head of a Pin?Molecular & cellular proteomics : MCP · 2016Article
- Diversity of the RFamide Peptide Family in Mollusks.Frontiers in endocrinology · 2014Review
- Small-volume analysis of cell-cell signaling molecules in the brain.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2014Review
- A multi-scale strategy for discovery of novel endogenous neuropeptides in the crustacean nervous system.Journal of proteomics · 2013Article
- High-definition de novo sequencing of crustacean hyperglycemic hormone (CHH)-family neuropeptides.Molecular & cellular proteomics : MCP · 2012Article
- The neuronal control of cardiac functions in Molluscs.Comparative biochemistry and physiology. Part A, Molecular & integrative physiology · 2011Review
- Proteomics in bone research.Expert review of proteomics · 2010Article
- Motility and flagellar glycosylation in Clostridium difficile.Journal of bacteriology · 2009Article
- Quantitative measurements of cell-cell signaling peptides with single-cell MALDI MS.Analytical chemistry · 2008Article
- Recent advances in proteomics and cancer biomarker discovery.Clinical medicine. Oncology · 2008Article
- Imaging mass spectrometry of neuropeptides in decapod crustacean neuronal tissues.Journal of proteome research · 2007Article
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11 authors.
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Abstract
In contrast to classical transmitters, the detailed structures and cellular and synaptic actions of neuropeptides are less well described. Peptide mass profiling of single identified neurons of the mollusc Lymnaea stagnalis indicated the presence of 17 abundant neuropeptides in the cardiorespiratory neuron, visceral dorsal 1 (VD1), and a subset of 14 peptides in its electrically coupled counterpart, right parietal dorsal 2. Altogether, based on this and previous work, we showed that the high number of peptides arises from the expression and processing of four distinct peptide precursor proteins, including a novel one. Second, we established a variety of posttranslational modifications of the generated peptides, including phosphorylation, disulphide linkage, glycosylation, hydroxylation, N-terminal pyroglutamylation, and C-terminal amidation. Specific synapses between VD1 and its muscle targets were formed, and their synaptic physiology was investigated. Whole-cell voltage-clamp analysis of dissociated heart muscle cells revealed, as tested for a selection of representative family members and their modifications, that the peptides of VD1 exhibit convergent activation of a high-voltage-activated Ca current. Moreover, the differentially glycosylated and hydroxylated alpha2 peptides were more potent than the unmodified alpha2 peptide in enhancing these currents. Together, this study is the first to demonstrate that single neurons exhibit such a complex pattern of peptide gene expression, precursor processing, and differential peptide modifications along with a remarkable degree of convergence of neuromodulatory actions. This study thus underscores the importance of a detailed mass spectrometric analysis of neuronal peptide content and peptide modifications related to neuromodulatory function.
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