ArticlePlant physiology2006
Glutamine synthetase-glutamate synthase pathway and glutamate dehydrogenase play distinct roles in the sink-source nitrogen cycle in tobacco.
Article in Plant physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 115 papers.
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115 citing papers in PubMed, 408 citations in OpenAlex.
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- Effects of High Temperature and Nitrogen Fertilizer on the Carbon and Nitrogen Metabolism Characteristics of Rice Varieties with Differing Taste Stability.Plants (Basel, Switzerland) · 2026Article
- Genome-wide characterization and transcriptional profiling of the glycerol-3-phosphate acyltransferase (GPAT) family in barley reveals the role of HvGPAT1 in low nitrogen tolerance.BMC plant biology · 2026Article
- Transcriptional regulation modulates nitrate uptake and utilization ofFrontiers in plant science · 2026Article
- Foliar application of licorice-wolfberry derived nanomaterials enhances soybean heat tolerance through maintaining reactive oxygen species homeostasis.Frontiers in plant science · 2026Article
- Expression of a constitutively active nitrate reductase increases SARS-CoV-2 Spike protein production in Nicotiana benthamiana leaves that otherwise show traits of senescence.Plant biotechnology journal · 2026Article
- Sulfur improvements in growth, nutrient use efficiency, and photosynthesis depend on ammonium-nitrate nutrition inFrontiers in plant science · 2026Article
- Dynamic multi-omics analysis reveals the correlation between aroma compounds and symbiotic microbial community during tobacco leaf aging process.BMC plant biology · 2025Article
- Regulatory Mechanisms of Free Umami Amino Acid Accumulation in Fresh Waxy Kernels: Insights from Transcriptome and Metabolomics Analyses.Foods (Basel, Switzerland) · 2025Article
- High-resolution time-series transcriptomic and metabolomic profiling reveals the regulatory mechanism underlying salt tolerance in maize.Genome biology · 2025Article
- Nitrogen-Driven Orchestration of Lateral Root Development: Molecular Mechanisms and Systemic Integration.Biology · 2025Review
- Microbially mediated silicon-based agro-wastes: a possible option in reducing bioaccumulation of arsenic.Frontiers in nutrition · 2025Review
- Proline Tagging for Stress Tolerance in Plants.International journal of genomics · 2025Review
- Glutamine Synthetase and Glutamate Synthase Family Perform Diverse Physiological Functions in Exogenous Hormones and Abiotic Stress Responses inPlants (Basel, Switzerland) · 2024Article
- Identification of Wheat Glutamate Synthetase Gene Family and Expression Analysis under Nitrogen Stress.Genes · 2024Article
- Physiological, molecular, and environmental insights into plant nitrogen uptake, and metabolism under abiotic stresses.The plant genome · 2024Review
- Chiral herbicide imazethapy influences plant-soil feedback on nitrogen metabolism by shaping rhizosphere microorganisms.Environmental science and pollution research international · 2024Article
- Comprehensive dissection of variation and accumulation of free amino acids in tea accessions.Horticulture research · 2024Article
55 more citing papers are in PubMed but not listed here.
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10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glutamate (Glu) metabolism and amino acid translocation were investigated in the young and old leaves of tobacco (Nicotiana tabacum L. cv Xanthi) using [15N]ammonium and [2-15N]Glu tracers. Regardless of leaf age, [15N]ammonium assimilation occurred via glutamine synthetase (GS; EC 6.1.1.3) and Glu synthase (ferredoxin [Fd]-GOGAT; EC 1.4.7.1; NADH-GOGAT; EC 1.4.1.14), both in the light and darkness, and it did not depend on Glu dehydrogenase (GDH; EC 1.4.1.2). The [15N]ammonium and ammonium accumulation patterns support the role of GDH in the deamination of [2-15N]Glu to provide 2-oxoglutarate and [15N]ammonium. In the dark, excess [15N]ammonium was incorporated into asparagine that served as an additional detoxification molecule. The constant Glu levels in the phloem sap suggested that Glu was continuously synthesized and supplied into the phloem regardless of leaf age. Further study using transgenic tobacco lines, harboring the promoter of the GLU1 gene (encoding Arabidopsis [Arabidopsis thaliana] Fd-GOGAT) fused to a GUS reporter gene, revealed that the expression of Fd-GOGAT remained higher in young leaves compared to old leaves, and higher in the veins compared to the mesophyll. Confocal laser-scanning microscopy localized the Fd-GOGAT protein to the phloem companion cells-sieve element complex in the leaf veins. The results are consistent with a role of Fd-GOGAT in supplying Glu for the synthesis and transport of amino acids. Taken together, the data provide evidence that the GS-GOGAT pathway and GDH play distinct roles in the source-sink nitrogen cycle of tobacco leaves.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.