ArticleMolecular biology of the cell2006
Conditional dominant mutations in the Caenorhabditis elegans gene act-2 identify cytoplasmic and muscle roles for a redundant actin isoform.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 47 citations in OpenAlex.
- Multiscale characterization ofiScience · 2025Article
- Functional fluorescence labeling of actins in live-cell system using engineered split-GFP technology.Molecular biology of the cell · 2025Article
- A sperm-oocyte protein partnership required for egg activation in Caenorhabditis elegans.Development (Cambridge, England) · 2025Article
- Transcriptomic Analysis of the Spatiotemporal Axis of Oogenesis and Fertilization inbioRxiv : the preprint server for biology · 2024Article
- Transcriptomic analysis of the spatiotemporal axis of oogenesis and fertilization inFrontiers in cell and developmental biology · 2024Article
- Contractile ring mechanosensation and its anillin-dependent tuning during early embryogenesis.Nature communications · 2023Article
- Branched-chain actin dynamics polarizes vesicle trajectories and partitions apicobasal epithelial membrane domains.Science advances · 2023Article
- A transient apical extracellular matrix relays cytoskeletal patterns to shape permanent acellular ridges on the surface of adult C. elegans.PLoS genetics · 2022Article
- Imaging of Actin Cytoskeleton in the Nematode Caenorhabditis elegans.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Single-cell RNA profiling links ncRNAs to spatiotemporal gene expression during C. elegans embryogenesis.Scientific reports · 2020Article
- F-Actin Cytoskeleton Network Self-Organization Through Competition and Cooperation.Annual review of cell and developmental biology · 2020Review
- Actomyosin contractility regulators stabilize the cytoplasmic bridge between the two primordial germ cells duringMolecular biology of the cell · 2017Article
- PAR proteins regulate maintenance-phase myosin dynamics duringMolecular biology of the cell · 2017Article
- Cortical forces and CDC-42 control clustering of PAR proteins for Caenorhabditis elegans embryonic polarization.Nature cell biology · 2017Article
- Regulation of structure and function of sarcomeric actin filaments in striated muscle of the nematode Caenorhabditis elegans.Anatomical record (Hoboken, N.J. : 2007) · 2014Review
- Systematic genetic interaction screens uncover cell polarity regulators and functional redundancy.Nature cell biology · 2013Article
- Article
- large-scale screening for targeted knockouts in the Caenorhabditis elegans genome.G3 (Bethesda, Md.) · 2012Article
- Smooth muscle α actin is specifically required for the maintenance of lactation.Developmental biology · 2012Article
- Biochemical and cell biological analysis of actin in the nematode Caenorhabditis elegans.Methods (San Diego, Calif.) · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Animal genomes each encode multiple highly conserved actin isoforms that polymerize to form the microfilament cytoskeleton. Previous studies of vertebrates and invertebrates have shown that many actin isoforms are restricted to either nonmuscle (cytoplasmic) functions, or to myofibril force generation in muscle cells. We have identified two temperature-sensitive and semidominant embryonic-lethal Caenorhabditis elegans mutants, each with a single mis-sense mutation in act-2, one of five C. elegans genes that encode actin isoforms. These mutations alter conserved and adjacent amino acids predicted to form part of the ATP binding pocket of actin. At the restrictive temperature, both mutations resulted in aberrant distributions of cortical microfilaments associated with abnormal and striking membrane ingressions and protrusions. In contrast to the defects caused by these dominant mis-sense mutations, an act-2 deletion did not result in early embryonic cell division defects, suggesting that additional and redundant actin isoforms are involved. Accordingly, we found that two additional actin isoforms, act-1 and act-3, were required redundantly with act-2 for cytoplasmic function in early embryonic cells. The act-1 and -3 genes also have been implicated previously in muscle function. We found that an ACT-2::GFP reporter was expressed cytoplasmically in embryonic cells and also was incorporated into contractile filaments in adult muscle cells. Furthermore, one of the dominant act-2 mutations resulted in uncoordinated adult movement. We conclude that redundant C. elegans actin isoforms function in both muscle and nonmuscle contractile processes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.