ArticleNucleic acids research2006
A novel multifunctional factor involved in trans-splicing of chloroplast introns in Chlamydomonas.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 80 citations in OpenAlex.
- Searching for α-solenoid proteins involved in organellar gene expression.BMC genomics · 2026Article
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- Plant organellar RNA maturation.The Plant cell · 2023Review
- The ChloroplastCells · 2021Review
- Pas de Trois: An Overview of Penta-, Tetra-, and Octo-Tricopeptide Repeat Proteins FromFrontiers in plant science · 2021Review
- The OPR Protein MTHI1 Controls the Expression of Two Different Subunits of ATP Synthase CFo inThe Plant cell · 2020Article
- PPR14 Interacts With PPR-SMR1 and CRM Protein Zm-mCSF1 to Facilitate Mitochondrial Intron Splicing in Maize.Frontiers in plant science · 2020Article
- Identification of clustered organellar short (cos) RNAs and of a conserved family of organellar RNA-binding proteins, the heptatricopeptide repeat proteins, in the malaria parasite.Nucleic acids research · 2018Article
- The FASTK family of proteins: emerging regulators of mitochondrial RNA biology.Nucleic acids research · 2017Review
- Small RNA profiling in Chlamydomonas: insights into chloroplast RNA metabolism.Nucleic acids research · 2017Article
- Programmable Genome Editing Tools and their Regulation for Efficient Genome Engineering.Computational and structural biotechnology journal · 2017Review
- Chloroplast genome expansion by intron multiplication in the basal psychrophilic euglenoidPeerJ · 2017Article
- CCS2, an Octatricopeptide-Repeat Protein, Is Required for Plastid CytochromeFrontiers in plant science · 2017Article
- A Ribonucleoprotein Supercomplex Involved in trans-Splicing of Organelle Group II Introns.The Journal of biological chemistry · 2016Article
- Using Group II Introns for Attenuating the In Vitro and In Vivo Expression of a Homing Endonuclease.PloS one · 2016Article
- Article
- The Octatricopeptide Repeat Protein Raa8 Is Required for Chloroplast trans Splicing.Eukaryotic cell · 2015Article
- Spontaneous dominant mutations in chlamydomonas highlight ongoing evolution by gene diversification.The Plant cell · 2015Article
- A nucleus-encoded chloroplast protein regulated by iron availability governs expression of the photosystem I subunit PsaA in Chlamydomonas reinhardtii.Plant physiology · 2015Article
- RAP, the sole octotricopeptide repeat protein in Arabidopsis, is required for chloroplast 16S rRNA maturation.The Plant cell · 2014Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the chloroplast of Chlamydomonas reinhardtii, psaA mRNA is spliced in trans from three separate precursors which assemble to form two group II introns. A fourth transcript, tscA, completes the structure of the first intron. Of the fourteen nucleus-encoded factors involved in psaA splicing, only two are required for splicing of both introns. We cloned and characterized the first of these more general factors, Raa1. Consistently with its role in psaA splicing, Raa1 is imported in the chloroplast where it is found in a membrane fraction and is part of a large ribonucleoprotein complex. One mutant, raa1-L137H, is defective for splicing of both introns, but another allelic mutant, raa1-314B, still expresses the 3' part of the Raa1 gene and is deficient only in splicing of intron 2. This observation and a deletion analysis indicate the presence of two domains in Raa1. The C-terminal domain is necessary and sufficient for processing of tscA RNA and splicing of the first intron, while the central domain is essential for splicing of the second intron. The combination of these two functional domains in Raa1 suggests that this new factor may coordinate trans-splicing of the two introns to improve the efficiency of psaA maturation.
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