ArticleNucleic acids research2006
Rapid quantitative profiling of complex microbial populations.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
69 citing papers in PubMed.
- Multiplex detection and identification of viral, bacterial, and protozoan pathogens in human blood and plasma using an expanded high-density resequencing microarray platform.Frontiers in molecular biosciences · 2024Article
- Considerations for the design and conduct of human gut microbiota intervention studies relating to foods.European journal of nutrition · 2020Review
- Emerging priorities for microbial metagenome research.Bioresource technology reports · 2020Review
- Acid Mine Drainage as Habitats for Distinct Microbiomes: Current Knowledge in the Era of Molecular and Omic Technologies.Current microbiology · 2020Review
- Clinical metagenomics.Nature reviews. Genetics · 2019Review
- Metagenomic investigation of gastrointestinal microbiome in cattle.Asian-Australasian journal of animal sciences · 2017Review
- Design and validation of a DNA-microarray for phylogenetic analysis of bacterial communities in different oral samples and dental implants.Scientific reports · 2017Article
- Tube-Wise Diagnostic Microarray for the Multiplex Characterization of the Complex Plant PathogenFrontiers in plant science · 2017Article
- Essential oils affect populations of some rumen bacteria in vitro as revealed by microarray (RumenBactArray) analysis.Frontiers in microbiology · 2015Article
- PhylOPDb: a 16S rRNA oligonucleotide probe database for prokaryotic identification.Database : the journal of biological databases and curation · 2014Article
- Gut microbial diversity in rat model induced by rhubarb.Experimental animals · 2014Article
- The potential link between gut microbiota and IgE-mediated food allergy in early life.International journal of environmental research and public health · 2013Review
- Comprehensive analysis of prokaryotes in environmental water using DNA microarray analysis and whole genome amplification.Pathogens (Basel, Switzerland) · 2013Article
- GPR-Analyzer: a simple tool for quantitative analysis of hierarchical multispecies microarrays.Environmental science and pollution research international · 2013Article
- Altered innate defenses in the neonatal gastrointestinal tract in response to colonization by neuropathogenic Escherichia coli.Infection and immunity · 2013Article
- Characterization of TEM-1 β-Lactamase-Producing Kingella kingae Clinical Isolates.Antimicrobial agents and chemotherapy · 2013Article
- The role of gut microbiota in the gut-brain axis: current challenges and perspectives.Protein & cell · 2013Review
- Gene capture coupled to high-throughput sequencing as a strategy for targeted metagenome exploration.DNA research : an international journal for rapid publication of reports on genes and genomes · 2013Article
- Upper gastrointestinal microbiota and digestive diseases.World journal of gastroenterology · 2013Review
- Microbial diversity in the era of omic technologies.BioMed research international · 2013Review
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Diverse and complex microbial ecosystems are found in virtually every environment on earth, yet we know very little about their composition and ecology. Comprehensive identification and quantification of the constituents of these microbial communities--a 'census'--is an essential foundation for understanding their biology. To address this problem, we developed, tested and optimized a DNA oligonucleotide microarray composed of 10,462 small subunit (SSU) ribosomal DNA (rDNA) probes (7167 unique sequences) selected to provide quantitative information on the taxonomic composition of diverse microbial populations. Using our optimized experimental approach, this microarray enabled detection and quantification of individual bacterial species present at fractional abundances of <0.1% in complex synthetic mixtures. The estimates of bacterial species abundance obtained using this microarray are similar to those obtained by phylogenetic analysis of SSU rDNA sequences from the same samples--the current 'gold standard' method for profiling microbial communities. Furthermore, probes designed to represent higher order taxonomic groups of bacterial species reliably detected microbes for which there were no species-specific probes. This simple, rapid microarray procedure can be used to explore and systematically characterize complex microbial communities, such as those found within the human body.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.