ArticleThe Journal of biological chemistry2006
Group V secretory phospholipase A2 translocates to the phagosome after zymosan stimulation of mouse peritoneal macrophages and regulates phagocytosis.
Article in The Journal of biological chemistry, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 114 citations in OpenAlex.
- Incorporation of Hydroxyeicosatetraenoic Acid Isomers into Macrophage Phospholipids Reveals Class-Specific Distribution.Biomolecules · 2026Article
- Secreted phospholipase A2 regulates intercellular communications by coordinating extracellular phospholipid metabolism.International immunology · 2025Review
- Bioactive lipid signaling and lipidomics in macrophage polarization: Impact on inflammation and immune regulation.Frontiers in immunology · 2025Review
- Differential Mobilization of the Phospholipid and Triacylglycerol Pools of Arachidonic Acid in Murine Macrophages.Biomolecules · 2022Article
- Characterization of the binding of cytosolic phospholipase AMolecular biology reports · 2022Article
- Group V Secretory Phospholipase AJournal of atherosclerosis and thrombosis · 2022Article
- Multimodal regulation of the osteoclastogenesis process by secreted group IIA phospholipase AFrontiers in cell and developmental biology · 2022Article
- Group V Phospholipase ACells · 2021Article
- The Roles of Phospholipase AFrontiers in cell and developmental biology · 2021Review
- Inflammation and JNK's Role in Niacin-GPR109A Diminished Flushed Effect in Microglial and Neuronal Cells With Relevance to Schizophrenia.Frontiers in psychiatry · 2021Review
- Lipid Profile of Activated Macrophages and Contribution of Group V Phospholipase ABiomolecules · 2020Article
- A Lipidomic Perspective of the Action of Group IIA Secreted Phospholipase ABiomolecules · 2020Article
- Exosomal miRNAs: novel players in viral infection.Epigenomics · 2020Review
- Harmful and protective roles of group V phospholipase ABiochimica et biophysica acta. Molecular and cell biology of lipids · 2019Review
- Prostaglandins in asthma and allergic diseases.Pharmacology & therapeutics · 2019Review
- Macrophages regulate lung ILC2 activation via Pla2g5-dependent mechanisms.Mucosal immunology · 2018Article
- Regulation of Phagocytosis in Macrophages by Membrane Ethanolamine Plasmalogens.Frontiers in immunology · 2018Article
- EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis.Methods in molecular biology (Clifton, N.J.) · 2017Article
- PLA2G5 regulates transglutaminase activity of human IL-4-activated M2 macrophages through PGE2 generation.Journal of leukocyte biology · 2016Article
- Group V Secretory Phospholipase A2 Is Involved in Tubular Integrity and Sodium Handling in the Kidney.PloS one · 2016Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
We have previously reported that group V secretory phospholipase A2 (sPLA2) amplifies the action of cytosolic phospholipase A2(cPLA2) alpha in regulating eicosanoid biosynthesis by mouse peritoneal macrophages stimulated with zymosan (Satake, Y., Diaz, B. L., Balestrieri, B., Lam, B. K., Kanaoka, Y., Grusby, M. J., and Arm, J. P. (2004) J. Biol. Chem. 279, 16488-16494). To further understand the role of group V sPLA2, we studied its localization in resting mouse peritoneal macrophages before and after stimulation with zymosan and the effect of deletion of the gene encoding group V sPLA2 on phagocytosis of zymosan. We report that group V sPLA2 is present in the Golgi apparatus and recycling endosome in the juxtanuclear region of resting peritoneal macrophages. Upon ingestion of zymosan by mouse peritoneal macrophages, group V sPLA2 is recruited to the phagosome. There it co-localizes with cPLA2alpha, 5-lipoxygenase, 5-lipoxygenase-activating protein, and leukotriene C4 synthase. Using immunostaining for the cysteinyl leukotrienes in carbodiimide-fixed cells, we show, for the first time, that the phagosome is a site of cysteinyl leukotriene formation. Furthermore, peritoneal macrophages from group V sPLA2-null mice demonstrated a >50% attenuation in phagocytosis of zymosan particles, which was restored by adenoviral expression of group V sPLA2 but IIA not group sPLA2. These data demonstrate that group V sPLA2 contributes to the innate immune response both through regulation of eicosanoid generation in response to a phagocytic stimulus and also as a component of the phagocytic machinery.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.