ArticlePlanta2006
Transgenic rice expressing Allium sativum leaf lectin with enhanced resistance against sap-sucking insect pests.
Article in Planta, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 142 citations in OpenAlex.
- Retraction Note: A novel approach for developing resistance in rice against phloem limited viruses by antagonizing the phloem feeding hemipteran vectors.Plant molecular biology · 2025Article
- Plant immunity to insect herbivores: mechanisms, interactions, and innovations for sustainable pest management.Frontiers in plant science · 2025Review
- Genome-wide selection of potential target candidates for RNAi against Nilaparvata lugens.BMC genomics · 2024Article
- Review
- Intein-mediated splitFrontiers in genome editing · 2024Article
- Plant Secondary Metabolites: The Weapons for Biotic Stress Management.Metabolites · 2023Review
- A novel plant lectin, NTL-125, interferes with SARS-CoV-2 interaction with hACE2.Virus research · 2022Article
- Article
- Research advances and prospects of legume lectins.Journal of biosciences · 2021Review
- 130 years of Plant Lectin Research.Glycoconjugate journal · 2020Review
- An efficient Agrobacterium-mediated genetic transformation method for foxtail millet (Setaria italica L.).Plant cell reports · 2020Article
- Changes in grapes composition during ripening of fivePhysiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- No Effect of Bt-transgenic Rice on the Tritrophic Interaction of the Stored Rice, the Maize Weevil Sitophilus Zeamais and the Parasitoid Wasp Theocolax elegans.Scientific reports · 2019Article
- Isolation of five rice nonendosperm tissue-expressed promoters and evaluation of their activities in transgenic rice.Plant biotechnology journal · 2018Article
- Expression of hybrid fusion protein (Cry1Ac::ASAL) in transgenic rice plants imparts resistance against multiple insect pests.Scientific reports · 2018Article
- Characterization of an Insecticidal Protein from Withania somnifera Against Lepidopteran and Hemipteran Pest.Molecular biotechnology · 2018Article
- A transgenic approach to control hemipteran insects by expressing insecticidal genes under phloem-specific promoters.Scientific reports · 2016Article
- Monitoring the efficacy of mutated Allium sativum leaf lectin in transgenic rice against Rhizoctonia solani.BMC biotechnology · 2016Article
- Engineered chloroplast dsRNA silences cytochrome p450 monooxygenase, V-ATPase and chitin synthase genes in the insect gut and disrupts Helicoverpa zea larval development and pupation.Plant biotechnology journal · 2015Article
- Temporal and spatial control of gene expression in horticultural crops.Horticulture research · 2014Review
Corrections and comments
- Expression of concern
- Retracted
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mannose binding Allium sativum leaf agglutinin (ASAL) has been shown to be antifeedant and insecticidal against sap-sucking insects. In the present investigation, ASAL coding sequence was expressed under the control of CaMV35S promoter in a chimeric gene cassette containing plant selection marker, hpt and gusA reporter gene of pCAMBIA1301 binary vector in an elite indica rice cv. IR64. Many fertile transgenic plants were generated using scutellar calli as initial explants through Agrobacterium-mediated transformation technology. GUS activity was observed in selected calli and in mature plants. Transformation frequency was calculated to be approximately 12.1%+/-0.351 (mean +/- SE). Southern blot analyses revealed the integration of ASAL gene into rice genome with a predominant single copy insertion. Transgene localization was detected on chromosomes of transformed plants using PRINS and C-PRINS techniques. Northern and western blot analyses determined the expression of transgene in transformed lines. ELISA analyses estimated ASAL expression up to 0.72 and 0.67% of total soluble protein in T0 and T1 plants, respectively. Survival and fecundity of brown planthopper and green leafhopper were reduced to 36% (P < 0.01), 32% (P < 0.05) and 40.5, 29.5% (P < 0.001), respectively, when tested on selected plants in comparison to control plants. Specific binding of expressed ASAL to receptor proteins of insect gut was analysed. Analysis of T1 progenies confirmed the inheritance of the transgenes. Thus, ASAL promises to be a potential component in insect resistance rice breeding programme.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.