ArticlePlant physiology2006
Agroinjection of tomato fruits. A tool for rapid functional analysis of transgenes directly in fruit.
Article in Plant physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
75 citing papers in PubMed, 231 citations in OpenAlex.
- Article
- Airborne β-caryophyllene disrupts virus-vector mutualism by priming tomato defenses.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Improvement and application of vacuum-infiltration system in tomato.Horticulture research · 2024Article
- A rapid and efficient Agrobacterium-mediated transient transformation system in grape berries.Protoplasma · 2024Article
- Transient Expression Assay and Microscopic Observation in Kumquat Fruit.Bio-protocol · 2024Article
- Light quality regulates plant biomass and fruit quality through a photoreceptor-dependent HY5-LHC/CYCB module in tomato.Horticulture research · 2023Article
- Principles and practice of virus induced gene silencing for functional genomics in plants.Virus genes · 2023Review
- Novel R2R3 MYB transcription factors regulate anthocyanin synthesis in Aubergine tomato plants.BMC plant biology · 2023Article
- Characterization of prolycopene-accumulated TanJournal of biosciences · 2023Article
- A tomato HD-zip I transcription factor, VAHOX1, acts as a negative regulator of fruit ripening.Horticulture research · 2023Article
- Tomato-made edible COVID-19 vaccine TOMAVAC induces neutralizing IgGs in the blood sera of mice and humans.Frontiers in nutrition · 2023Article
- A comparative transcriptomics and eQTL approach identifies SlWD40 as a tomato fruit ripening regulator.Plant physiology · 2022Article
- Unique features of mRNA m6A methylomes during expansion of tomato (Solanum lycopersicum) fruits.Plant physiology · 2022Article
- Plant biomacromolecule delivery methods in the 21st century.Frontiers in genome editing · 2022Review
- Multi-Omics Analysis Reveals ThatFrontiers in plant science · 2022Article
- A novel elicitor protein phosphopentomutase fromFrontiers in plant science · 2022Article
- Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants.International journal of molecular sciences · 2021Review
- Illuminating the cells: transient transformation of citrus to study gene functions and organelle activities related to fruit quality.Horticulture research · 2021Article
- An improved and efficient method of Agrobacterium syringe infiltration for transient transformation and its application in the elucidation of gene function in poplar.BMC plant biology · 2021Article
- A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants.Frontiers in plant science · 2021Review
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transient expression of foreign genes in plant tissues is a valuable tool for plant biotechnology. To shorten the time for gene functional analysis in fruits, we developed a transient methodology that could be applied to tomato (Solanum lycopersicum cv Micro Tom) fruits. It was found that injection of Agrobacterium cultures through the fruit stylar apex resulted in complete fruit infiltration. This infiltration method, named fruit agroinjection, rendered high levels of 35S Cauliflower mosaic virus-driven beta-glucuronidase and yellow fluorescence protein transient expression in the fruit, with higher expression levels around the placenta and moderate levels in the pericarp. Usefulness of fruit agroinjection was assayed in three case studies: (1) the heat shock regulation of an Arabidopsis (Arabidopsis thaliana) promoter, (2) the production of recombinant IgA antibodies as an example of molecular farming, and (3) the virus-induced gene silencing of the carotene biosynthesis pathway. In all three instances, this technology was shown to be efficient as a tool for fast transgene expression in fruits.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.