ArticleBiophysical journal2006
An alternative domain near the ATP binding pocket of Drosophila myosin affects muscle fiber kinetics.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 39 citations in OpenAlex.
- Structure of the Inhibited Smooth Muscle Myosin and Its Implications on the Regulation of Insect Striated Muscle Myosin.Life (Basel, Switzerland) · 2025Review
- A Drosophila cardiac myosin increases jump muscle stretch activation and shortening deactivation.Biophysical journal · 2025Article
- Rbfox1 is required for myofibril development and maintaining fiber type-specific isoform expression inLife science alliance · 2022Article
- X-ray Crystallographic and Molecular Dynamic Analyses of Drosophila melanogaster Embryonic Muscle Myosin Define Domains Responsible for Isoform-Specific Properties.Journal of molecular biology · 2020Article
- The load dependence of muscle's force-velocity curve is modulated by alternative myosin converter domains.American journal of physiology. Cell physiology · 2019Article
- Five Alternative Myosin Converter Domains Influence Muscle Power, Stretch Activation, and Kinetics.Biophysical journal · 2018Article
- Article
- An embryonic myosin converter domain influences Drosophila indirect flight muscle stretch activation, power generation and flight.The Journal of experimental biology · 2014Article
- To understand muscle you must take it apart.Frontiers in physiology · 2014Review
- Shared gene structures and clusters of mutually exclusive spliced exons within the metazoan muscle myosin heavy chain genes.PloS one · 2014Article
- An embryonic myosin isoform enables stretch activation and cyclical power in Drosophila jump muscle.Biophysical journal · 2013Article
- Calcium signalling indicates bilateral power balancing in the Drosophila flight muscle during manoeuvring flight.Journal of the Royal Society, Interface · 2013Article
- Function of alternative splicing.Gene · 2013Review
- Mechanical analysis of Drosophila indirect flight and jump muscles.Methods (San Diego, Calif.) · 2012Review
- Transgenic expression and purification of myosin isoforms using the Drosophila melanogaster indirect flight muscle system.Methods (San Diego, Calif.) · 2012Article
- Deletion of Drosophila muscle LIM protein decreases flight muscle stiffness and power generation.American journal of physiology. Cell physiology · 2011Article
- Distribution of myosin attachment times predicted from viscoelastic mechanics of striated muscle.Journal of biomedicine & biotechnology · 2011Article
- The influence of myosin converter and relay domains on cross-bridge kinetics of Drosophila indirect flight muscle.Biophysical journal · 2010Article
- Mutating the converter-relay interface of Drosophila myosin perturbs ATPase activity, actin motility, myofibril stability and flight ability.Journal of molecular biology · 2010Article
- The mechanical properties of Drosophila jump muscle expressing wild-type and embryonic Myosin isoforms.Biophysical journal · 2010Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
We examined the importance of alternative versions of a region near the ATP binding site of Drosophila myosin heavy chain for muscle mechanical properties. Previously, we exchanged two versions of this region (encoded by alternative exon 7s) between the indirect flight muscle myosin isoform (IFI) and an embryonic myosin isoform (EMB) and found, surprisingly, that in vitro solution actin-activated ATPase rates were increased (higher Vmax) by both exon exchanges. Here we examined the effect of increased ATPase rate on indirect flight muscle (IFM) fiber mechanics and Drosophila locomotion. IFM expressing EMB with the exon 7a domain replaced by the IFM specific exon 7d domain (EMB-7d) exhibited 3.2-fold greater maximum oscillatory power (Pmax) and 1.5-fold greater optimal frequency of power generation (fmax) versus fibers expressing EMB. In contrast, IFM expressing IFI with the exon 7d region replaced by the EMB exon 7a region (IFI-7a), showed no change in Pmax, fmax, step response, or isometric muscle properties compared to native IFI fibers. A slight decrement in IFI-7a flight ability was observed, suggesting a negative influence of the increased ATPase rate on Drosophila locomotion, perhaps due to energy supply constraints. Our results show that exon 7 plays a substantial role in establishing fiber speed and flight performance, and that the limiting step that sets ATPase rate in Drosophila myosin has little to no direct influence in setting fmax for fast muscle fiber types.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.