ArticleBiophysical journal2006
Distinct membrane mechanical properties of human mesenchymal stem cells determined using laser optical tweezers.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed, 136 citations in OpenAlex.
- At the Pointy End of Mechanobiology: AFM for Transient Biomechanical Analysis.Advanced healthcare materials · 2025Review
- Robust quantification of cellular mechanics using optical tweezers.Biophysical reports · 2025Article
- DNA methyltransferase 1 deficiency improves macrophage motility and wound healing by ameliorating cholesterol accumulation.NPJ Regenerative medicine · 2023Article
- Changes in the mechanical properties of human mesenchymal stem cells during differentiation.Royal Society open science · 2023Article
- Manipulation and Mechanical Deformation of Leukemia Cells by High-Frequency Ultrasound Single Beam.IEEE transactions on ultrasonics, ferroelectrics, and frequency control · 2022Article
- The Role of Cytoskeleton Revealed by Quartz Crystal Microbalance and Digital Holographic Microscopy.International journal of molecular sciences · 2022Article
- Multi-frequency passive and active microrheology with optical tweezers.Scientific reports · 2021Article
- How cholesterol stiffens unsaturated lipid membranes.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Experimental study of the difference in deformation between normal and pathological, renal and bladder, cells induced by acoustic radiation force.European biophysics journal : EBJ · 2020Article
- Cardiomyocyte mechanodynamics under conditions of actin remodelling.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Membrane cholesterol and substrate stiffness co-ordinate to induce the remodelling of the cytoskeleton and the alteration in the biomechanics of vascular smooth muscle cells.Cardiovascular research · 2019Article
- Mesenchymal Stem Cell Deformability and Implications for Microvascular Sequestration.Annals of biomedical engineering · 2018Article
- Biomechanical Characterization at the Cell Scale: Present and Prospects.Frontiers in physiology · 2018Review
- Cell Deformation by Single-beam Acoustic Trapping: A Promising Tool for Measurements of Cell Mechanics.Scientific reports · 2016Article
- Nanomechanical and surface properties of rMSCs post-exposure to CAP treated UHMWPE wear particles.Nanomedicine : nanotechnology, biology, and medicine · 2016Article
- Myogenic and neurogenic differentiation of human tooth germ stem cells (hTGSCs) are regulated by pluronic block copolymers.Cytotechnology · 2016Article
- Quantifying Molecular-Level Cell Adhesion on Electroactive Conducting Polymers using Electrochemical-Single Cell Force Spectroscopy.Scientific reports · 2015Article
- High-Throughput Assessment of Cellular Mechanical Properties.Annual review of biomedical engineering · 2015Review
- Stem Cells toward the Future: The Space Challenge.Life (Basel, Switzerland) · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The therapeutic efficacy of mesenchymal stem cells (MSCs) in tissue engineering and regenerative medicine is determined by their unique biological, mechanical, and physicochemical characteristics, which are yet to be fully explored. Cell membrane mechanics, for example, has been shown to critically influence MSC differentiation. In this study, we used laser optical tweezers to measure the membrane mechanics of human MSCs and terminally differentiated fibroblasts by extracting tethers from the outer cell membrane. The average tether lengths were 10.6+/-1.1 microm (hMSC) and 3.0+/-0.5 microm (fibroblasts). The tether extraction force did not increase during tether formation, which suggests existence of a membrane reservoir intended to buffer membrane tension fluctuations. Cytoskeleton disruption resulted in a fourfold tether length increase in fibroblasts but had no effect in hMSCs, indicating weak association between the cell membrane and hMSC actin cytoskeleton. Cholesterol depletion, known to decrease lipid bilayer stiffness, caused an increase in the tether length both in fibroblasts and hMSCs, as does the treatment of cells with DMSO. We postulate that whereas fibroblasts use both the membrane rigidity and membrane-cytoskeleton association to regulate their membrane reservoir, hMSC cytoskeleton has only a minor impact on stem cell membrane mechanics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.