ArticleThe Journal of cell biology1992
Calmodulin concentrates at regions of cell growth in Saccharomyces cerevisiae.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 91 citations in OpenAlex.
- A conserved fungal hub protein involved in adhesion and drug resistance in the human pathogenCell surface (Amsterdam, Netherlands) · 2018Article
- From networks of protein interactions to networks of functional dependencies.BMC systems biology · 2012Article
- An intein with genetically selectable markers provides a new approach to internally label proteins with GFP.BMC biotechnology · 2011Article
- Calmodulin dissociation regulates Myo5 recruitment and function at endocytic sites.The EMBO journal · 2010Article
- Calmodulin concentrates at the apex of growing hyphae and localizes to the Spitzenkörper in Aspergillus nidulans.Protoplasma · 2006Article
- A type V myosin (Myo2p) and a Rab-like G-protein (Ypt11p) are required for retention of newly inherited mitochondria in yeast cells during cell division.Molecular biology of the cell · 2004Article
- Sla1p is a functionally modular component of the yeast cortical actin cytoskeleton required for correct localization of both Rho1p-GTPase and Sla2p, a protein with talin homology.Molecular biology of the cell · 1999Article
- The multiple roles of Cyk1p in the assembly and function of the actomyosin ring in budding yeast.Molecular biology of the cell · 1999Article
- The yeast centrin, cdc31p, and the interacting protein kinase, Kic1p, are required for cell integrity.The Journal of cell biology · 1998Article
- Mlc1p is a light chain for the unconventional myosin Myo2p in Saccharomyces cerevisiae.The Journal of cell biology · 1998Article
- Iqg1p, a yeast homologue of the mammalian IQGAPs, mediates cdc42p effects on the actin cytoskeleton.The Journal of cell biology · 1998Article
- Article
- High rates of actin filament turnover in budding yeast and roles for actin in establishment and maintenance of cell polarity revealed using the actin inhibitor latrunculin-A.The Journal of cell biology · 1997Article
- Ca2+-calmodulin promotes survival of pheromone-induced growth arrest by activation of calcineurin and Ca2+-calmodulin-dependent protein kinase.Molecular and cellular biology · 1996Article
- The BUD4 protein of yeast, required for axial budding, is localized to the mother/BUD neck in a cell cycle-dependent manner.The Journal of cell biology · 1996Article
- Role of calmodulin and Spc110p interaction in the proper assembly of spindle pole body compenents.The Journal of cell biology · 1996Article
- end5, end6, and end7: mutations that cause actin delocalization and block the internalization step of endocytosis in Saccharomyces cerevisiae.Molecular biology of the cell · 1995Article
- Molecular basis of cell integrity and morphogenesis in Saccharomyces cerevisiae.Microbiological reviews · 1995Review
- AFR1 promotes polarized apical morphogenesis in Saccharomyces cerevisiae.Molecular and cellular biology · 1995Article
- Calcium-independent calmodulin requirement for endocytosis in yeast.The EMBO journal · 1994Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Calmodulin was localized in Saccharomyces cerevisiae by indirect immunofluorescence using affinity-purified polyclonal antibodies. Calmodulin displays an asymmetric distribution that changes during the cell cycle. In unbudded cells, calmodulin concentrates at the presumptive site of bud formation approximately 10 min before bud emergence. In small budded cells, calmodulin accumulates throughout the bud. As the bud grows, calmodulin concentrates at the tip, then disperses, and finally concentrates in the neck region before cytokinesis. An identical staining pattern is observed when wild-type calmodulin is replaced with mutant forms of calmodulin impaired in binding Ca2+. Thus, the localization of calmodulin does not depend on its ability to bind Ca2+ with a high affinity. Double labeling of yeast cells with affinity-purified anti-calmodulin antibody and rhodamine-conjugated phalloidin indicates that calmodulin and actin concentrate in overlapping regions during the cell cycle. Furthermore, disrupting calmodulin function using a temperature-sensitive calmodulin mutant delocalizes actin, and act1-4 mutants contain a random calmodulin distribution. Thus, calmodulin and actin distributions are interdependent. Finally, calmodulin localizes to the shmoo tip in cells treated with alpha-factor. This distribution, at sites of cell growth, implicates calmodulin in polarized cell growth in yeast.
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