ArticleThe Journal of cell biology1992
A temperature-sensitive calmodulin mutant loses viability during mitosis.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 78 citations in OpenAlex.
- Calmodulin and Its Interactive Proteins Participate in Regulating the Explosive Growth ofInternational journal of molecular sciences · 2021Article
- Lessons from yeast: the spindle pole body and the centrosome.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Ca(2+) signaling, genes and the cell cycle.Cell calcium · 2010Review
- Phosphorylation of the chromosomal passenger protein Bir1 is required for localization of Ndc10 to the spindle during anaphase and full spindle elongation.Molecular biology of the cell · 2006Article
- The nuclear pore complex-associated protein, Mlp2p, binds to the yeast spindle pole body and promotes its efficient assembly.The Journal of cell biology · 2005Article
- The Saccharomyces cerevisiae spindle pole body is a dynamic structure.Molecular biology of the cell · 2003Article
- The yeast class V myosins, Myo2p and Myo4p, are nonprocessive actin-based motors.The Journal of cell biology · 2001Article
- Yeast Mps1p phosphorylates the spindle pole component Spc110p in the N-terminal domain.The Journal of biological chemistry · 2001Article
- Mlc1p is a light chain for the unconventional myosin Myo2p in Saccharomyces cerevisiae.The Journal of cell biology · 1998Article
- Iqg1p, a yeast homologue of the mammalian IQGAPs, mediates cdc42p effects on the actin cytoskeleton.The Journal of cell biology · 1998Article
- A mutational analysis identifies three functional regions of the spindle pole component Spc110p in Saccharomyces cerevisiae.Molecular biology of the cell · 1997Article
- Spc110p: assembly properties and role in the connection of nuclear microtubules to the yeast spindle pole body.The EMBO journal · 1996Article
- Role of calmodulin and Spc110p interaction in the proper assembly of spindle pole body compenents.The Journal of cell biology · 1996Article
- The 110-kD spindle pole body component of Saccharomyces cerevisiae is a phosphoprotein that is modified in a cell cycle-dependent manner.The Journal of cell biology · 1996Article
- Spc42p: a phosphorylated component of the S. cerevisiae spindle pole body (SPD) with an essential function during SPB duplication.The Journal of cell biology · 1996Article
- end5, end6, and end7: mutations that cause actin delocalization and block the internalization step of endocytosis in Saccharomyces cerevisiae.Molecular biology of the cell · 1995Article
- The PMR2 gene cluster encodes functionally distinct isoforms of a putative Na+ pump in the yeast plasma membrane.The EMBO journal · 1995Article
- Gain-of-function mutations in a human calmodulin-like protein identify residues critical for calmodulin action in yeast.Molecular & general genetics : MGG · 1995Article
- Calcium-independent calmodulin requirement for endocytosis in yeast.The EMBO journal · 1994Article
- The spindle pole body of yeast.Chromosoma · 1994Review
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Although rare, a recessive temperature-sensitive calmodulin mutant has been isolated in Saccharomyces cerevisiae. The mutant carries two mutations in CMD1, isoleucine 100 is changed to asparagine and glutamic acid 104 is changed to valine. Neither mutation alone conferred temperature sensitivity. A single mutation that allowed production of an intact but defective protein was not identified. At the nonpermissive temperature, the temperature-sensitive mutant displayed multiple defects. Bud formation and growth was delayed, but this defect was not responsible for the temperature-sensitive lethality. Cells synchronized in G1 progressed through the cell cycle and retained viability until the movement of the nucleus to the neck between the mother cell and the large bud. After nuclear movement, less than 5% of the cells survived the first mitosis and could form colonies when returned to permissive conditions. The duplicated DNA was dispersed along the spindle, extending from mother to daughter cell. Cells synchronized in G2/M lost viability immediately upon the shift to the nonpermissive temperature. At a semipermissive temperature, the mutant showed approximately a 10-fold increase in the rate of chromosome loss compared to a wild-type strain. The mitotic phenotype is very similar to yeast mutants that are defective in chromosome disjunction. The mutant also showed defects in cytokinesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.