ArticleThe Biochemical journal2006
CpG methylation at the USF-binding site is important for the liver-specific transcription of the chipmunk HP-27 gene.
Article in The Biochemical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 59 citations in OpenAlex.
- Periodic expression of Per1 gene is restored in chipmunk liver during interbout arousal in mammalian hibernation.Scientific reports · 2025Article
- The Whole Genome DNA Methylation Signatures of Hindlimb Muscles in Chinese Alligators during Hibernation and Active Periods.Animals : an open access journal from MDPI · 2024Article
- Liver transcriptomic and methylomic analyses identify transcriptional mitogen-activated protein kinase regulation in facultative hibernation of Syrian hamster.Proceedings. Biological sciences · 2023Article
- Effect of MAOA DNA Methylation on Human in Vivo Protein Expression Measured by [11C]harmine Positron Emission Tomography.The international journal of neuropsychopharmacology · 2023Article
- Big brown bats experience slower epigenetic ageing during hibernation.Proceedings. Biological sciences · 2022Article
- Epigenetic regulation by DNA methyltransferases during torpor in the thirteen-lined ground squirrel Ictidomys tridecemlineatus.Molecular and cellular biochemistry · 2021Article
- DNA Methylation of PigGenes · 2021Article
- A Unique Energy-Saving Strategy during Hibernation Revealed by Multi-Omics Analysis in the Chinese Alligator.iScience · 2020Article
- Ectopic Methylation of a Single Persistently Unmethylated CpG in the Promoter of the Vitellogenin Gene Abolishes Its Inducibility by Estrogen through Attenuation of Upstream Stimulating Factor Binding.Molecular and cellular biology · 2019Article
- Circadian transcription factor HSF1 regulates differential HSP70 gene transcription during the arousal-torpor cycle in mammalian hibernation.Scientific reports · 2019Article
- HNF-4 participates in the hibernation-associated transcriptional regulation of the chipmunk hibernation-related protein gene.Scientific reports · 2017Article
- The dynamic nature of DNA methylation: a role in response to social and seasonal variation.Integrative and comparative biology · 2014Review
- Seasonality modifies methylation profiles in healthy people.PloS one · 2014Article
- Reintroducing domesticated wild mice to sociality induces adaptive transgenerational effects on MUP expression.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- DNA methylation analyses of urothelial carcinoma reveal distinct epigenetic subtypes and an association between gene copy number and methylation status.Epigenetics · 2012Article
- DNA demethylation and USF regulate the meiosis-specific expression of the mouse Miwi.PLoS genetics · 2012Article
- DNA methylation of the gonadal aromatase (cyp19a) promoter is involved in temperature-dependent sex ratio shifts in the European sea bass.PLoS genetics · 2011Article
- Potential utility of natural products as regulators of breast cancer-associated aromatase promoters.Reproductive biology and endocrinology : RB&E · 2011Review
- Epigenetic regulation of the calcitonin gene-related peptide gene in trigeminal glia.Cephalalgia : an international journal of headache · 2011Article
- Distinct DNA methylation patterns characterize differentiated human embryonic stem cells and developing human fetal liver.Genome research · 2009Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chipmunk hibernation-specific HP-27 gene is expressed specifically in the liver and has a CpG-poor promoter. To reveal how the liver-specific transcription of the HP-27 gene is regulated, we performed yeast one-hybrid screening of a chipmunk liver cDNA library. A 5'-flanking sequence of the HP-27 gene, extending from -170 to -140 and containing an E-box (5'-CACGTG-3'), is essential for the liver-specific transcription of HP-27. We used this sequence as bait and found that a ubiquitously expressed transcription factor, USF (upstream stimulatory factor), bound to the E-box. In COS-7 cells, USF activated transcription from the HP-27 gene promoter. We then used bisulphite genomic sequencing to analyse the methylation status of the four CpG dinucleotides that lie in the 5'-flanking sequence of the HP-27 gene up to -450, to investigate how the ubiquitously expressed USF activates transcription of the HP-27 gene only in the liver, while its transcription is repressed elsewhere. The only difference in methylation in the tissues tested was in the CpG dinucleotide in the USF-binding site, which was hypomethylated in the liver, but highly methylated in the kidney and heart. The specific methylation of the CpG dinucleotide at the USF-binding site impeded both the binding of USF and its transcriptional activation of the HP-27 gene. Chromatin immunoprecipitation using anti-USF antibodies revealed that USF bound to the HP-27 gene promoter in the liver, but not in the kidney or heart. Thus CpG methylation at the USF-binding site functions in establishing and maintaining tissue-specific transcription from the CpG-poor HP-27 gene promoter.
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