ArticlePlanta2006
Sialic acid concentrations in plants are in the range of inadvertent contamination.
Article in Planta, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 108 citations in OpenAlex.
- Changes in Metabolites Produced in Wheat Plants Against Water-Deficit Stress.Plants (Basel, Switzerland) · 2024Article
- Putative rhamnogalacturonan-II glycosyltransferase identified through callus gene editing which bypasses embryo lethality.Plant physiology · 2024Article
- Harnessing Plant Sugar Metabolism for Glycoengineering.Biology · 2023Article
- Heat Stress of Algal Partner Hinders Colonization Success and Alters the Algal Cell Surface Glycome in a Cnidarian-Algal Symbiosis.Microbiology spectrum · 2022Article
- From simple and specific zymographic detections to the annotation of a fungus Daldinia caldariorum D263 that encodes a wide range of highly bioactive cellulolytic enzymes.Biotechnology for biofuels · 2021Article
- Origin and evolution of nonulosonic acid synthases and their relationship with bacterial pathogenicity revealed by a large-scale phylogenetic analysis.Microbial genomics · 2021Article
- Glyco-Engineering of Plant-Based Expression Systems.Advances in biochemical engineering/biotechnology · 2021Article
- Tackling the chemical diversity of microbial nonulosonic acids - a universal large-scale survey approach.Chemical science · 2020Article
- Stable Protein Sialylation in Physcomitrella.Frontiers in plant science · 2020Article
- A Roadmap for the Molecular Farming of Viral Glycoprotein Vaccines: Engineering Glycosylation and Glycosylation-Directed Folding.Frontiers in plant science · 2020Review
- Nucleotide Sugar Transporter SLC35 Family Structure and Function.Computational and structural biotechnology journal · 2019Review
- Serum sialylation changes in cancer.Glycoconjugate journal · 2018Review
- Reconstruction of the sialylation pathway in the ancestor of eukaryotes.Scientific reports · 2018Article
- Ribosome Inactivating Proteins from Rosaceae.Molecules (Basel, Switzerland) · 2016Article
- Endoplasmic reticulum: Where nucleotide sugar transport meets cytokinin control mechanisms.Plant signaling & behavior · 2015Article
- Expression of recombinant vaccines and antibodies in plants.Monoclonal antibodies in immunodiagnosis and immunotherapy · 2014Review
- Ubiquitous sialometabolism present among oral fusobacteria.PloS one · 2014Article
- Protein N-glycosylation in eukaryotic microalgae and its impact on the production of nuclear expressed biopharmaceuticals.Frontiers in plant science · 2014Review
- Porcine sialoadhesin: a newly identified xenogeneic innate immune receptor.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2012Article
- Engineering of sialylated mucin-type O-glycosylation in plants.The Journal of biological chemistry · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The long held but challenged view that plants do not synthesize sialic acids was re-evaluated using two different procedures to isolate putative sialic acid containing material from plant tissues and cells. The extracts were reacted with 1,2-diamino-4,5-methylene dioxybenzene and the fluorescently labelled 2-keto sugar acids analysed by reversed phase and normal phase HPLC and by HPLC-electrospray tandem mass spectrometry. No N-glycolylneuraminic acid was found in the protein fraction from Arabidopsis thaliana MM2d cells. However, we did detect 3-deoxy-D: -manno-octulosonic acid and trace amounts (3-18 pmol/g fresh weight) of a compound indistinguishable from N-acetylneuraminic acid by its retention time and its mass spectral fragmentation pattern. Thus, plant cells and tissues contain five orders of magnitude less sialic acid than mammalian tissues such as porcine liver. Similar or lower amounts of N-acetylneuraminic acid were detected in tobacco cells, mung bean sprouts, apple and banana. Yet even yeast and buffer blanks, when subjected to the same isolation procedures, apparently contained the equivalent of 5 pmol of sialic acid per gram of material. Thus, we conclude that it is not possible to demonstrate unequivocally that plants synthesize sialic acids because the amounts of these sugars detected in plant cells and tissues are so small that they may originate from extraneous contaminants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.