ArticleMolecular biology of the cell2006
Protein kinase Cgamma regulates myosin IIB phosphorylation, cellular localization, and filament assembly.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 54 citations in OpenAlex.
- Scribble, Lgl1, and myosin IIA interact with α-/β-catenin to maintain epithelial junction integrity.Cell adhesion & migration · 2023Article
- Scribble, Lgl1, and myosin II form a complex in vivo to promote directed cell migration.Molecular biology of the cell · 2020Article
- The Cytoskeleton of the Retinal Pigment Epithelium: from Normal Aging to Age-Related Macular Degeneration.International journal of molecular sciences · 2019Review
- The Cytoskeleton-A Complex Interacting Meshwork.Cells · 2019Review
- Spatio-Temporal Regulation of RhoGTPases Signaling by Myosin II.Frontiers in cell and developmental biology · 2019Review
- Common and Specific Functions of Nonmuscle Myosin II Paralogs in Cells.Biochemistry. Biokhimiia · 2018Review
- Mammalian nonmuscle myosin II comes in three flavors.Biochemical and biophysical research communications · 2018Review
- Article
- aPKCζ affects directed cell migration through the regulation of myosin light chain phosphorylation.Cell adhesion & migration · 2017Article
- Myosin IIA Heavy Chain Phosphorylation Mediates Adhesion Maturation and Protrusion in Three Dimensions.The Journal of biological chemistry · 2017Article
- A regulatory motif in nonmuscle myosin II-B regulates its role in migratory front-back polarity.The Journal of cell biology · 2015Article
- Endogenous species of mammalian nonmuscle myosin IIA and IIB include activated monomers and heteropolymers.Current biology : CB · 2014Article
- Non-muscle myosin II regulates neuronal actin dynamics by interacting with guanine nucleotide exchange factors.PloS one · 2014Article
- High resolution characterization of myosin IIC protein tailpiece and its effect on filament assembly.The Journal of biological chemistry · 2013Article
- Neuroprotective effects of resveratrol and epigallocatechin gallate polyphenols are mediated by the activation of protein kinase C gamma.Frontiers in cellular neuroscience · 2013Article
- The tumor suppressor Lgl1 regulates NMII-A cellular distribution and focal adhesion morphology to optimize cell migration.Molecular biology of the cell · 2012Article
- Article
- Myosin II isoform switching mediates invasiveness after TGF-β-induced epithelial-mesenchymal transition.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- A cascade of protein kinase C isozymes promotes cytoskeletal polarization in T cells.Nature immunology · 2011Article
- Determinants of myosin II cortical localization during cytokinesis.Current biology : CB · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nonmuscle myosin II is an important component of the cytoskeleton, playing a major role in cell motility and chemotaxis. We have previously demonstrated that, on stimulation with epidermal growth factor (EGF), nonmuscle myosin heavy chain II-B (NMHC-IIB) undergoes a transient phosphorylation correlating with its cellular localization. We also showed that members of the PKC family are involved in this phosphorylation. Here we demonstrate that of the two conventional PKC isoforms expressed by prostate cancer cells, PKCbetaII and PKCgamma, PKCgamma directly phosphorylates NMHC-IIB. Overexpression of wild-type and kinase dead dominant negative PKCgamma result in both altered NMHC-IIB phosphorylation and subcellular localization. We have also mapped the phosphorylation sites of PKCgamma on NMHC-IIB. Conversion of the PKCgamma phosphorylation sites to alanine residues, reduces the EGF-dependent NMHC-IIB phosphorylation. Aspartate substitution of these sites reduces NMHC-IIB localization into cytoskeleton. These results indicate that PKCgamma regulates NMHC-IIB phosphorylation and cellular localization in response to EGF stimulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.