ArticleMolecular biology of the cell2006
Actin-based motility during endocytosis in budding yeast.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 62 citations in OpenAlex.
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- Cytosolic concentrations of actin binding proteins and the implications for in vivo F-actin turnover.The Journal of cell biology · 2023Article
- Evolutionary tuning of barbed end competition allows simultaneous construction of architecturally distinct actin structures.The Journal of cell biology · 2023Article
- Proteomic Markers for Mechanobiological Properties of Metastatic Cancer Cells.International journal of molecular sciences · 2023Review
- Optimizing microtubule arrangements for rapid cargo capture.Biophysical journal · 2021Article
- Capping Protein Insulates Arp2/3-Assembled Actin Patches from Formins.Current biology : CB · 2019Article
- "Essentially, all models are wrong, but some are useful"-a cross-disciplinary agenda for building useful models in cell biology and biophysics.Biophysical reviews · 2018Review
- Actin organization and endocytic trafficking are controlled by a network linking NIMA-related kinases to the CDC-42-SID-3/ACK1 pathway.PLoS genetics · 2018Article
- MoCAP proteins regulated by MoArk1-mediated phosphorylation coordinate endocytosis and actin dynamics to govern development and virulence of Magnaporthe oryzae.PLoS genetics · 2017Article
- TORC2 and eisosomes are spatially interdependent, requiring optimal level of phosphatidylinositol 4, 5-bisphosphate for their integrity.Journal of biosciences · 2015Article
- Actin and endocytosis in budding yeast.Genetics · 2015Review
- Identification and characterization of Rvs162/Rvs167-3, a novel N-BAR heterodimer in the human fungal pathogen Candida albicans.Eukaryotic cell · 2015Article
- Coordination of the filament stabilizing versus destabilizing activities of cofilin through its secondary binding site on actin.Cytoskeleton (Hoboken, N.J.) · 2014Article
- Force generation by endocytic actin patches in budding yeast.Biophysical journal · 2014Article
- Zooming in on the molecular mechanisms of endocytic budding by time-resolved electron microscopy.Cellular and molecular life sciences : CMLS · 2014Review
- Inactivation of Tor proteins affects the dynamics of endocytic proteins in early stage of endocytosis.Journal of biosciences · 2013Article
- Ultrastructural imaging of endocytic sites in Saccharomyces cerevisiae by transmission electron microscopy and immunolabeling.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2013Article
- Ultrastructural dynamics of proteins involved in endocytic budding.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Capping protein modulates the dynamic behavior of actin filaments in response to phosphatidic acid in Arabidopsis.The Plant cell · 2012Article
- Cdk1-dependent control of membrane-trafficking dynamics.Molecular biology of the cell · 2012Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Actin assembly nucleated by Arp2/3 complex has been implicated in the formation and movement of endocytic vesicles. The dendritic nucleation model has been proposed to account for Arp2/3-mediated actin assembly and movement. Here, we explored the model by examining the role of capping protein in vivo, with quantitative tracking analysis of fluorescence markers for different stages of endocytosis in yeast. Capping protein was most important for the initial movement of endocytic vesicles away from the plasma membrane, which presumably corresponds to vesicle scission and release. The next phase of endosome movement away from the plasma membrane was also affected, but less so. The results are consistent with the dendritic nucleation model's prediction of capping protein as important for efficient actin assembly and force production. In contrast, the movement of late-stage endocytic vesicles, traveling through the cytoplasm en route to the vacuole, did not depend on capping protein. The movement of these vesicles was found previously to depend on Lsb6, a WASp interactor, whereas Lsb6 was found here to be dispensable for early endosome movement. Thus, the molecular requirements for Arp2/3-based actin assembly differ in early versus later stages of endocytosis. Finally, acute loss of actin cables led to increased patch motility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.