ArticleApplied and environmental microbiology2006
Fluorescence in situ hybridization using peptide nucleic acid probes for rapid detection of Mycobacterium avium subsp. avium and Mycobacterium avium subsp. paratuberculosis in potable-water biofilms.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 60 citations in OpenAlex.
- The zoonotic potential of Mycobacterium avium spp. paratuberculosis: a systematic review.Canadian journal of public health = Revue canadienne de sante publiquePooled it
- Use of peptide nucleic acid fluorescent in situ hybridization technique in the detection of Mycobacterium avium paratuberculosis in fecal samples from dairy herds.Veterinary research communications · 2026Article
- Genital mycobacteriosis caused by Mycobacterium marinum detected in two captive sharks by peptide nucleic acid-fluorescence in situ hybridization.Journal of fish diseases · 2023Article
- Development of a Novel Peptide Nucleic Acid Probe for the Detection ofMicroorganisms · 2022Article
- Potential of Flow Cytometric Approaches for Rapid Microbial Detection and Characterization in the Food Industry-A Review.Foods (Basel, Switzerland) · 2021Review
- Non-Tuberculous Mycobacteria: Molecular and Physiological Bases of Virulence and Adaptation to Ecological Niches.Microorganisms · 2020Review
- Article
- Quantification of Mycobacterium avium subspecies in pig tissues by real-time quantitative PCR.Acta veterinaria Scandinavica · 2013Article
- Molecular survey of the occurrence of Legionella spp., Mycobacterium spp., Pseudomonas aeruginosa, and amoeba hosts in two chloraminated drinking water distribution systems.Applied and environmental microbiology · 2012Article
- PNA-FISH as a new diagnostic method for the determination of clarithromycin resistance of Helicobacter pylori.BMC microbiology · 2011Article
- Interaction of Legionella pneumophila and Helicobacter pylori with bacterial species isolated from drinking water biofilms.BMC microbiology · 2011Article
- DNA mimics for the rapid identification of microorganisms by fluorescence in situ hybridization (FISH).International journal of molecular sciences · 2008Article
- Persistence of Helicobacter pylori in heterotrophic drinking-water biofilms.Applied and environmental microbiology · 2008Article
- Survival of Mycobacterium avium in drinking water biofilms as affected by water flow velocity, availability of phosphorus, and temperature.Applied and environmental microbiology · 2007Article
- Development and application of a novel peptide nucleic acid probe for the specific detection of Helicobacter pylori in gastric biopsy specimens.Journal of clinical microbiology · 2007Article
- Survival of Mycobacterium avium, Legionella pneumophila, Escherichia coli, and caliciviruses in drinking water-associated biofilms grown under high-shear turbulent flow.Applied and environmental microbiology · 2007Article
- Evaluation of peptide nucleic acid-fluorescence in situ hybridization for identification of clinically relevant mycobacteria in clinical specimens and tissue sections.Journal of clinical microbiology · 2006Article
- Targeting species-specific low-affinity 16S rRNA binding sites by using peptide nucleic acids for detection of Legionellae in biofilms.Applied and environmental microbiology · 2006Article
- Evaluation of in situ methods used to detect Mycobacterium avium subsp. paratuberculosis in samples from patients with Crohn's disease.Journal of clinical microbiology · 2006Article
- Permeabilization of the mycobacterial envelope for protein cytolocalization studies by immunofluorescence microscopy.BMC microbiology · 2006Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we present for the first time a high-affinity peptide nucleic acid (PNA) oligonucleotide sequence for detecting Mycobacterium avium bacteria, including the opportunistically pathogenic subspecies M. avium subsp. avium, M. avium subsp. paratuberculosis, and M. avium subsp. silvaticum, by the fluorescence in situ hybridization (FISH) method. There is evidence that M. avium subsp. avium especially is able to survive and grow in drinking-water biofilms and possibly transmit via drinking water. The designed PNA probe (MAV148) specificity was tested with several bacterial species, including other mycobacteria and mycolic acid-containing bacteria. From the range of bacterial strains tested, only M. avium subsp. avium and M. avium subsp. paratuberculosis strains were hybridized. The PNA FISH method was applied successfully to detect M. avium subsp. avium spiked in water samples and biofilm established within a Propella biofilm reactor fed with potable water from a distribution supply.
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