ArticleApplied and environmental microbiology2006
Real-time fluorogenic reverse transcription-PCR assays for detection of bacteriophage MS2.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 117 citations in OpenAlex.
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- Flow-Based Dielectrophoretic Biosensor for Detection of Bacteriophage MS2 as a Foodborne Virus Surrogate.Biosensors · 2025Article
- Evaluating the capacity of magnetic ionic liquids for separation and concentration of non-enveloped viral particles and free viral genomic RNA.Analytical and bioanalytical chemistry · 2025Article
- Sampling efficiency and nucleic acid stability during long-term sampling with different bioaerosol samplers.Environmental monitoring and assessment · 2024Article
- A novel strategy to avoid sensitivity loss in pooled testing for SARS-CoV-2 surveillance: validation using nasopharyngeal swab and saliva samples.Frontiers in public health · 2023Article
- Simple Affinity-Based Method for Concentrating Viruses from Wastewater Using Engineered Curli Fibers.ACS ES&T water · 2022Article
- Applicability Evaluation of Male-Specific Coliphage-Based Detection Methods for Microbial Contamination Tracking.Journal of microbiology and biotechnology · 2021Article
- Bacteriophages as Fecal Pollution Indicators.Viruses · 2021Review
- Optimization and Implementation of the Virus Extraction Method for Hepatitis E Virus Detection from Raw Pork Liver.Food and environmental virology · 2021Article
- Comparison of samplers collecting airborne influenza viruses: 1. Primarily impingers and cyclones.PloS one · 2021Article
- Discovery of Components Acting as the Obstacles in the Detection of Enteric Viruses from Berries.Food and environmental virology · 2020Article
- Evaluation of Virus Reduction by Ultrafiltration with Coagulation-Sedimentation in Water Reclamation.Food and environmental virology · 2017Article
- Field-deployable, quantitative, rapid identification of active Ebola virus infection in unprocessed blood.Chemical science · 2017Article
- Assessing microbial decontamination of indoor air with particular focus on human pathogenic viruses.American journal of infection control · 2016Review
- Resistance of Aerosolized Bacterial Viruses to Relative Humidity and Temperature.Applied and environmental microbiology · 2015Article
- Enzymatic Pre-treatment of Wastewater to Minimize Recovery by Reverse Transcriptase PCR of RNA from Inactive Bacteriophages.Current microbiology · 2015Article
- Evaluating efficacy of field-generated electrochemical oxidants on disinfection of fomites using bacteriophage MS2 and mouse norovirus MNV-1 as pathogenic virus surrogates.Food and environmental virology · 2014Article
- Survival of airborne MS2 bacteriophage generated from human saliva, artificial saliva, and cell culture medium.Applied and environmental microbiology · 2014Article
- Short RNA indicator sequences are not completely degraded by autoclaving.Scientific reports · 2014Article
- Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteriophage MS2 is used in place of pathogenic viruses in a wide variety of studies that range from testing of compounds for disinfecting surfaces to studying environmental transport and fate of pathogenic viruses in groundwater. MS2 is also used as a pathogen simulant in the research, development, and testing (including open air tests) of methods, systems, and devices for the detection of pathogens in both the battlefield and homeland defense settings. PCR is often used as either an integral part of such detection systems or as a reference method to assess the sensitivity and specificity of microbial detection. To facilitate the detection of MS2 by PCR, we describe here a set of real-time fluorogenic reverse transcription-PCR assays. The sensitivity of the assays (performed with primer pairs and corresponding dye-labeled probes) ranged from 0.4 to 40 fg of MS2 genomic RNA (200 to 20,000 genome equivalents). We also demonstrate the usefulness of the primer pairs in assays without dye-labeled probe that included the DNA-binding dye SYBR green. None of the assays gave false-positive results when tested against 400 pg of several non-MS2 nucleic acid targets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.