ArticleApplied and environmental microbiology2006
Efficient and stable display of functional proteins on bacterial magnetic particles using mms13 as a novel anchor molecule.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Rapid and sensitive detection of glucocorticoids using engineered magnetosomes functionalized protein A conjugated broad-spectrum monoclonal antibody.Food chemistry: X · 2024Article
- Fusion expression of nanobodies specific for the insecticide fipronil on magnetosomes in Magnetospirillum gryphiswaldense MSR-1.Journal of nanobiotechnology · 2021Article
- Artificial coiled coil biomineralisation protein for the synthesis of magnetic nanoparticles.Nature communications · 2019Article
- Magnetosome biogenesis in magnetotactic bacteria.Nature reviews. Microbiology · 2016Review
- From invagination to navigation: The story of magnetosome-associated proteins in magnetotactic bacteria.Protein science : a publication of the Protein Society · 2016Review
- Functional expression of full-length TrkA in the prokaryotic host Magnetospirillum magneticum AMB-1 by using a magnetosome display system.Applied and environmental microbiology · 2015Article
- Article
- Functional expression of thyroid-stimulating hormone receptor on nano-sized bacterial magnetic particles in Magnetospirillum magneticum AMB-1.International journal of molecular sciences · 2013Article
- The MagA protein of Magnetospirilla is not involved in bacterial magnetite biomineralization.Journal of bacteriology · 2012Article
- Relevant uses of surface proteins--display on self-organized biological structures.Microbial biotechnology · 2012Review
- Magnetosome expression of functional camelid antibody fragments (nanobodies) in Magnetospirillum gryphiswaldense.Applied and environmental microbiology · 2011Article
- In vivo biotinylation of bacterial magnetic particles by a truncated form of Escherichia coli biotin ligase and biotin acceptor peptide.Applied and environmental microbiology · 2010Article
- Inducible expression of transmembrane proteins on bacterial magnetic particles in Magnetospirillum magneticum AMB-1.Applied and environmental microbiology · 2010Article
- In vivo display of a multisubunit enzyme complex on biogenic magnetic nanoparticles.Applied and environmental microbiology · 2009Article
- Identification of promoters for efficient gene expression in Magnetospirillum gryphiswaldense.Applied and environmental microbiology · 2009Article
- Production, Modification and Bio-Applications of Magnetic Nanoparticles Gestated by Magnetotactic Bacteria.Nano research · 2009Article
- Formation of magnetite by bacteria and its application.Journal of the Royal Society, Interface · 2008Review
- Noncovalent immobilization of streptavidin on in vitro- and in vivo-biotinylated bacterial magnetic particles.Applied and environmental microbiology · 2008Article
- Expression of green fluorescent protein fused to magnetosome proteins in microaerophilic magnetotactic bacteria.Applied and environmental microbiology · 2008Article
- Development of a cell surface display system in a magnetotactic bacterium, "Magnetospirillum magneticum" AMB-1.Applied and environmental microbiology · 2008Article
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2 authors.
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Abstract
Magnetic particles are increasingly used for various biomedical applications because they are easy to handle and separate from biological samples. In this work, a novel anchor molecule was used for targeted protein display onto magnetic nanoparticles. The magnetic bacterium Magnetospirillum magneticum AMB-1 synthesizes intracellular bacterial magnetic particles (BMPs) covered with a lipid bilayer membrane. In our recent research, an integral BMP membrane protein, Mms13, was isolated and used as an anchor molecule to display functional proteins onto BMPs. The anchoring properties of Mms13 were confirmed by luciferase fusion studies. The C terminus of Mms13 was shown to be expressed on the surface of BMPs, and Mms13 was bound to magnetite directly and tightly permitting stable localization of a large protein, luciferase (61 kDa), on BMPs. Consequently, luminescence intensity obtained from BMPs using Mms13 as an anchor molecule was >400 or 1,000 times higher than Mms16 or MagA, which previously were used as anchor molecules. Furthermore, the immunoglobulin G-binding domain of protein A (ZZ) was displayed uniformly on BMPs using Mms13, and antigen was detected by transmission electron microscopy using antibody-labeled gold nanoparticles on a single BMP displaying the ZZ-antibody complex. The results of this study demonstrated the utility of Mms13 as a molecular anchor, which will facilitate the assembly of other functional proteins onto BMPs in the near feature.
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