ArticleApplied and environmental microbiology2006
Isolation of poly-3-hydroxybutyrate metabolism genes from complex microbial communities by phenotypic complementation of bacterial mutants.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Article
- The transport of mannitol inMicrobiology (Reading, England) · 2023Article
- Characterization of Mutations That Affect the Nonoxidative Pentose Phosphate Pathway in Sinorhizobium meliloti.Journal of bacteriology · 2018Article
- Functional metagenomics reveals novel β-galactosidases not predictable from gene sequences.PloS one · 2017Article
- Synthetic biology approaches to improve biocatalyst identification in metagenomic library screening.Microbial biotechnology · 2015Review
- Plant-microbe Cross-talk in the Rhizosphere: Insight and Biotechnological Potential.The open microbiology journal · 2015Article
- Evaluation of a pooled strategy for high-throughput sequencing of cosmid clones from metagenomic libraries.PloS one · 2014Article
- Bioprospecting potential of the soil metagenome: novel enzymes and bioactivities.Genomics & informatics · 2013Review
- Open resource metagenomics: a model for sharing metagenomic libraries.Standards in genomic sciences · 2011Article
- Isolation of xylose isomerases by sequence- and function-based screening from a soil metagenomic library.Biotechnology for biofuels · 2011Article
- Metagenomic analyses: past and future trends.Applied and environmental microbiology · 2011Review
- Size Does Matter: Application-driven Approaches for Soil Metagenomics.Soil biology & biochemistry · 2010Article
- Production of the chiral compound (R)-3-hydroxybutyrate by a genetically engineered methylotrophic bacterium.Applied and environmental microbiology · 2010Article
- Identification and characterization of new LuxR/LuxI-type quorum sensing systems from metagenomic libraries.Environmental microbiology · 2010Article
- Achievements and new knowledge unraveled by metagenomic approaches.Applied microbiology and biotechnology · 2009Review
- Rapid identification of genes encoding DNA polymerases by function-based screening of metagenomic libraries derived from glacial ice.Applied and environmental microbiology · 2009Article
- Influence of the poly-3-hydroxybutyrate (PHB) granule-associated proteins (PhaP1 and PhaP2) on PHB accumulation and symbiotic nitrogen fixation in Sinorhizobium meliloti Rm1021.Journal of bacteriology · 2007Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The goal of this study was to initiate investigation of the genetics of bacterial poly-3-hydroxybutyrate (PHB) metabolism at the community level. We constructed metagenome libraries from activated sludge and soil microbial communities in the broad-host-range IncP cosmid pRK7813. Several unique clones were isolated from these libraries by functional heterologous complementation of a Sinorhizobium meliloti bdhA mutant, which is unable to grow on the PHB cycle intermediate D-3-hydroxybutyrate due to absence of the enzyme D-3-hydroxybutyrate dehydrogenase activity. Clones that conferred D-3-hydroxybutyrate utilization on Escherichia coli were also isolated. Although many of the S. meliloti bdhA mutant complementing clones restored D-3-hydroxybutyrate dehydrogenase activity to the mutant host, for some of the clones this activity was not detectable. This was also the case for almost all of the clones isolated in the E. coli selection. Further analysis was carried out on clones isolated in the S. meliloti complementation. Transposon mutagenesis to locate the complementing genes, followed by DNA sequence analysis of three of the genes, revealed coding sequences that were broadly divergent but lay within the diversity of known short-chain dehydrogenase/reductase encoding genes. In some cases, the amino acid sequence identity between pairs of deduced BdhA proteins was <35%, a level at which detection by nucleic acid hybridization based methods would probably not be successful.
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