ArticleApplied and environmental microbiology2006
Long serial analysis of gene expression for gene discovery and transcriptome profiling in the widespread marine coccolithophore Emiliania huxleyi.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 85 citations in OpenAlex.
- Dealing with phosphorus deficiency: contrasting strategies in marine phytoplankton and bacteria.ISME communications · 2026Article
- Polyphosphate Polymerase-A Key Enzyme for the Phosphorus Economy of the Microalgal Cell and the Sustainable Usage of This Nutrient.Plants (Basel, Switzerland) · 2025Review
- Giant Virus Infection Signatures Are Modulated by Euphotic Zone Depth Strata and Iron Regimes of the Subantarctic Southern Ocean.mSystems · 2023Article
- Association of Phosphatidylinositol-Specific Phospholipase C with Calcium-Induced Biomineralization in the CoccolithophoreMicroorganisms · 2020Article
- Proteomic Profiling ofMolecules (Basel, Switzerland) · 2020Article
- Effect of KNOInternational journal of biological sciences · 2019Article
- Specialized proteomic responses and an ancient photoprotection mechanism sustain marine green algal growth during phosphate limitation.Nature microbiology · 2018Article
- Utilization of urea and expression profiles of related genes in the dinoflagellate Prorocentrum donghaiense.PloS one · 2017Article
- Conserved Transcriptional Responses to Nutrient Stress in Bloom-Forming Algae.Frontiers in microbiology · 2017Article
- Article
- Transcriptome Analysis of Scrippsiella trochoidea CCMP 3099 Reveals Physiological Changes Related to Nitrate Depletion.Frontiers in microbiology · 2016Article
- Functional group-specific traits drive phytoplankton dynamics in the oligotrophic ocean.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Metatranscriptome analyses indicate resource partitioning between diatoms in the field.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Changes in gene expression of Prymnesium parvum induced by nitrogen and phosphorus limitation.Frontiers in microbiology · 2015Article
- Emiliania huxleyi endures N-limitation with an efficient metabolic budgeting and effective ATP synthesis.BMC genomics · 2014Article
- De novo assembly of Aureococcus anophagefferens transcriptomes reveals diverse responses to the low nutrient and low light conditions present during blooms.Frontiers in microbiology · 2014Article
- Gene expression studies for the analysis of domoic acid production in the marine diatom Pseudo-nitzschia multiseries.BMC molecular biology · 2013Article
- The effect of nitrate and phosphate availability on Emiliania huxleyi (NZEH) physiology under different CO2 scenarios.Frontiers in microbiology · 2013Article
- Genome variations associated with viral susceptibility and calcification in Emiliania huxleyi.PloS one · 2013Article
- Characterization of the proteomic profiles of the brown tide alga Aureoumbra lagunensis under phosphate- and nitrogen-limiting conditions and of its phosphate limitation-specific protein with alkaline phosphatase activity.Applied and environmental microbiology · 2012Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The abundant and widespread coccolithophore Emiliania huxleyi plays an important role in mediating CO2 exchange between the ocean and the atmosphere through its impact on marine photosynthesis and calcification. Here, we use long serial analysis of gene expression (SAGE) to identify E. huxleyi genes responsive to nitrogen (N) or phosphorus (P) starvation. Long SAGE is an elegant approach for examining quantitative and comprehensive gene expression patterns without a priori knowledge of gene sequences via the detection of 21-bp nucleotide sequence tags. E. huxleyi appears to have a robust transcriptional-level response to macronutrient deficiency, with 42 tags uniquely present or up-regulated twofold or greater in the N-starved library and 128 tags uniquely present or up-regulated twofold or greater in the P-starved library. The expression patterns of several tags were validated with reverse transcriptase PCR. Roughly 48% of these differentially expressed tags could be mapped to publicly available genomic or expressed sequence tag (EST) sequence data. For example, in the P-starved library a number of the tags mapped to genes with a role in P scavenging, including a putative phosphate-repressible permease and a putative polyphosphate synthetase. In short, the long SAGE analyses have (i) identified many new differentially regulated gene sequences, (ii) assigned regulation data to EST sequences with no database homology and unknown function, and (iii) highlighted previously uncharacterized aspects of E. huxleyi N and P physiology. To this end, our long SAGE libraries provide a new public resource for gene discovery and transcriptional analysis in this biogeochemically important marine organism.
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