ArticleApplied and environmental microbiology2006
Occurrence and genetic diversity of uncultured Legionella spp. in drinking water treated at temperatures below 15 degrees C.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 117 citations in OpenAlex.
- Foresight 2035: a perspective on the next decade of research on the management of Legionella spp. in engineered aquatic environments.FEMS microbiology reviews · 2025Review
- Occurrence of UnculturedPathogens (Basel, Switzerland) · 2024Article
- Opportunistic Pathogens in Drinking Water Distribution Systems-A Review.Microorganisms · 2024Review
- How hydrolytic exoribonucleases impact human disease: Two sides of the same story.FEBS open bio · 2023Review
- Review
- Characterization of the First Cultured Psychrotolerant Representative ofMicrobiology spectrum · 2021Article
- Legionella and other opportunistic pathogens in full-scale chloraminated municipal drinking water distribution systems.Water research · 2021Article
- Quantification of Legionella pneumophila by qPCR and culture in tap water with different concentrations of residual disinfectants and heterotrophic bacteria.The Science of the total environment · 2021Article
- Comparison of two culture methods for the enumeration of Legionella pneumophila from potable water samples.Journal of water and health · 2021Article
- Chasing Waterborne Pathogens in Antarctic Human-Made and Natural Environments, with Special Reference toApplied and environmental microbiology · 2021Article
- Complete Genome Sequence of Novel PsychrotolerantMicrobiology resource announcements · 2020Article
- Characterization of bacterial communities in wastewater with enhanced taxonomic resolution by full-length 16S rRNA sequencing.Scientific reports · 2019Article
- Assessing the impact, genomics and evolution of type II secretion across a large, medically important genus: the Legionella type II secretion paradigm.Microbial genomics · 2019Article
- A Proactive Environmental Approach for Preventing Legionellosis in Infants: Water Sampling and Antibiotic Resistance Monitoring, a 3-Years Survey Program.Healthcare (Basel, Switzerland) · 2019Article
- The Water Microbiome Through a Pilot Scale Advanced Treatment Facility for Direct Potable Reuse.Frontiers in microbiology · 2019Article
- The all-intracellular order Legionellales is unexpectedly diverse, globally distributed and lowly abundant.FEMS microbiology ecology · 2018Article
- Detection of Pathogenic and Non-pathogenic Bacteria in Drinking Water and Associated Biofilms on the Crow Reservation, Montana, USA.Microbial ecology · 2018Article
- Effect of temperature and colonization of Legionella pneumophila and Vermamoeba vermiformis on bacterial community composition of copper drinking water biofilms.Microbial biotechnology · 2017Article
- Methodological approaches for monitoring opportunistic pathogens in premise plumbing: A review.Water research · 2017Review
- Annual variations and effects of temperature on Legionella spp. and other potential opportunistic pathogens in a bathroom.Environmental science and pollution research international · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Representatives of the genus Legionella were detected by use of a real-time PCR method in all water samples collected directly after treatment from 16 surface water (SW) supplies prior to postdisinfection and from 81 groundwater (GW) supplies. Legionella concentrations ranged from 1.1 x 10(3) to 7.8 x 10(5) cells liter(-1) and were significantly higher in SW treated with multiple barriers at 4 degrees C than in GW treated at 9 to 12 degrees C with aeration and filtration but without chemical disinfection. No Legionellae (<50 CFU liter(-1)) were detected in treated water by the culture method. Legionella was also observed in untreated SW and in untreated aerobic and anaerobic GW. Filtration processes in SW and GW treatment had little effect or increased the Legionella concentration, but ozonation in SW treatment caused about 1-log-unit reduction. A phylogenetic analysis of 16S rRNA gene sequences of 202 clones, obtained from a selection of samples, showed a high similarity (>91%) with Legionella sequences in the GenBank database. A total of 40 (33%) of the 16S rRNA gene sequences obtained from treated water were identified as described Legionella species and types, including L. bozemanii, L. worsleiensis, Legionella-like amoebal pathogen types, L. quateirensis, L. waltersii, and L. pneumophila. 16S rRNA gene sequences with a similarity of below 97% from described species were positioned all over the phylogenetic tree of Legionella. Hence, a large diversity of yet-uncultured Legionellae are common members of the microbial communities in SW and GW treated at water temperatures of below 15 degrees C.
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