ArticleApplied and environmental microbiology2006
Production of recombinant and tagged proteins in the hyperthermophilic archaeon Sulfolobus solfataricus.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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61 citing papers in PubMed, 139 citations in OpenAlex.
- Review
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- Mining thermophiles for biotechnologically relevant enzymes: evaluating the potential of European and Caucasian hot springs.Extremophiles : life under extreme conditions · 2023Review
- A temperature dependent pilin promoter for production of thermostable enzymes in Thermus thermophilus.Microbial cell factories · 2023Article
- The biology of thermoacidophilic archaea from the order Sulfolobales.FEMS microbiology reviews · 2021Article
- CRISPR-Cas adaptive immune systems in Sulfolobales: genetic studies and molecular mechanisms.Science China. Life sciences · 2021Review
- Engineering Biology to Construct Microbial Chassis for the Production of Difficult-to-Express Proteins.International journal of molecular sciences · 2020Review
- Identification of XylR, the Activator of Arabinose/Xylose Inducible Regulon inFrontiers in microbiology · 2020Article
- Purification and characterization of a novel thermophilic β-galactosidase from Picrophilus torridus of potential industrial application.Extremophiles : life under extreme conditions · 2019Article
- Ring nucleases deactivate type III CRISPR ribonucleases by degrading cyclic oligoadenylate.Nature · 2018Article
- Biotechnology of extremely thermophilic archaea.FEMS microbiology reviews · 2018Review
- Modification of translation factor aIF5A from Sulfolobus solfataricus.Extremophiles : life under extreme conditions · 2018Article
- Review: Engineering of thermostable enzymes for industrial applications.APL bioengineering · 2018Review
- In a quest for engineering acidophiles for biomining applications: challenges and opportunities.Genes · 2018Review
- Sulfolobus acidocaldarius Transports Pentoses via a Carbohydrate Uptake Transporter 2 (CUT2)-Type ABC Transporter and Metabolizes Them through the Aldolase-Independent Weimberg Pathway.Applied and environmental microbiology · 2018Article
- The SmAP2 RNA binding motif in the 3'UTR affects mRNA stability in the crenarchaeum Sulfolobus solfataricus.Nucleic acids research · 2017Article
- The SmAP1/2 proteins of the crenarchaeon Sulfolobus solfataricus interact with the exosome and stimulate A-rich tailing of transcripts.Nucleic acids research · 2017Article
- Review
- Repression of RNA polymerase by the archaeo-viral regulator ORF145/RIP.Nature communications · 2016Article
- Efficient CRISPR-Mediated Post-Transcriptional Gene Silencing in a Hyperthermophilic Archaeon Using Multiplexed crRNA Expression.G3 (Bethesda, Md.) · 2016Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many systems are available for the production of recombinant proteins in bacterial and eukaryotic model organisms, which allow us to study proteins in their native hosts and to identify protein-protein interaction partners. In contrast, only a few transformation systems have been developed for archaea, and no system for high-level gene expression existed for hyperthermophilic organisms. Recently, a virus-based shuttle vector with a reporter gene was developed for the crenarchaeote Sulfolobus solfataricus, a model organism of hyperthermophilic archaea that grows optimally at 80 degrees C (M. Jonuscheit, E. Martusewitsch, K. M. Stedman, and C. Schleper, Mol. Microbiol. 48:1241-1252, 2003). Here we have refined this system for high-level gene expression in S. solfataricus with the help of two different promoters, the heat-inducible promoter of the major chaperonin, thermophilic factor 55, and the arabinose-inducible promoter of the arabinose-binding protein AraS. Functional expression of heterologous and homologous genes was demonstrated, including production of the cytoplasmic sulfur oxygenase reductase from Acidianus ambivalens, an Fe-S protein of the ABC class from S. solfataricus, and two membrane-associated ATPases potentially involved in the secretion of proteins. Single-step purification of the proteins was obtained via fused His or Strep tags. To our knowledge, these are the first examples of the application of an expression vector system to produce large amounts of recombinant and also tagged proteins in a hyperthermophilic archaeon.
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