ArticleApplied and environmental microbiology2006
Sensitive determination of microbial growth by nucleic acid staining in aqueous suspension.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Surface sediment microbial communities remain viable, culturable, and metabolically active during sequential heating.Frontiers in microbiology · 2026Article
- Plastic additives enrich diverse bacterial communities which show the hallmarks of plastic degradation.Sustainable microbiology · 2026Article
- Isolation of a putative sulfur comproportionating microorganism.Scientific reports · 2025Article
- Tip extension and simultaneous multiple fission in a filamentous bacterium.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Flow cytometry-based viability staining: an at-line tool for bioprocess monitoring of Sulfolobus acidocaldarius.AMB Express · 2022Article
- Analysis of Polyhydroxyalkanoates Granules inPolymers · 2021Article
- Performance Evaluation of Biozentech Malaria Scanner in Plasmodium knowlesi and P. falciparum as a New Diagnostic Tool.The Korean journal of parasitology · 2021Article
- Accurate identification and quantification of commensal microbiota bound by host immunoglobulins.Microbiome · 2021Article
- Determination of live and dead Komagataeibacter xylinus cells and first attempt at precise control of inoculation in nanocellulose production.Microbial biotechnology · 2020Article
- Physiological Capabilities of Cryoconite Hole Microorganisms.Frontiers in microbiology · 2020Article
- A simple, fast and reliable scan-based technique as a novel approach to quantify intracellular bacteria.BMC microbiology · 2019Article
- High-Throughput Quantification of Bacterial-Cell Interactions Using Virtual Colony Counts.Frontiers in cellular and infection microbiology · 2018Article
- High abundance of ammonia-oxidizing Archaea in coastal waters, determined using a modified DNA extraction method.Applied and environmental microbiology · 2010Article
- Subsurface microbiology and biogeochemistry of a deep, cold-water carbonate mound from the Porcupine Seabight (IODP Expedition 307).Environmental microbiology · 2009Article
- Two distinct Photobacterium populations thrive in ancient Mediterranean sapropels.Microbial ecology · 2008Article
- Microbial diversity in coastal subsurface sediments: a cultivation approach using various electron acceptors and substrate gradients.Applied and environmental microbiology · 2005Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The determination of cell numbers or biomass in laboratory cultures or environmental samples is usually based on turbidity measurements, viable counts, biochemical determinations (e.g., protein and lipid measurements), microscopic counting, or recently, flow cytometric analysis. In the present study, we developed a novel procedure for the sensitive quantification of microbial cells in cultures and most-probable-number series. The assay combines fluorescent nucleic acid staining and subsequent fluorescence measurement in suspension. Six different fluorescent dyes (acridine orange, DAPI [4',6'-diamidino-2-phenylindole], ethidium bromide, PicoGreen, and SYBR green I and II) were evaluated. SYBR green I was found to be the most sensitive dye and allowed the quantification of 50,000 to up to 1.5 x 10(8) Escherichia coli cells per ml sample. The rapid staining procedure was robust against interference from rRNA, sample fixation by the addition of glutaric dialdehyde, and reducing agents such as sodium dithionite, sodium sulfide, and ferrous sulfide. It worked well with phylogenetically distant bacterial and archaeal strains. Excellent agreement with optical density measurements of cell increases was achieved during growth experiments performed with aerobic and sulfate-reducing bacteria. The assay offers a time-saving, more sensitive alternative to epifluorescence microscopy analysis of most-probable-number dilution series. This method simplifies the quantification of microbial cells in pure cultures as well as enrichments and is particularly suited for low cell densities.
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