ArticleApplied and environmental microbiology2006
Improvement of alpha-amylase production by modulation of ribosomal component protein S12 in Bacillus subtilis 168.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 54 citations in OpenAlex.
- Improvement of neutral protease activity ofJournal of Zhejiang University. Science. B · 2025Article
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- Mutations in the regulatory regions result in increased streptomycin resistance and keratinase synthesis in Bacillus thuringiensis.Archives of microbiology · 2021Article
- Ribosome-EngineeredApplied and environmental microbiology · 2020Article
- Engineering the Translational Machinery for Biotechnology Applications.Molecular biotechnology · 2020Review
- Genome shuffling for improving the activity of alkaline pectinase in Bacillus subtilis FS105 and its molecular mechanism.World journal of microbiology & biotechnology · 2019Article
- The Application of Ribosome Engineering to Natural Product Discovery and Yield Improvement inAntibiotics (Basel, Switzerland) · 2019Review
- Combinatorial Effect of ARTP Mutagenesis and Ribosome Engineering on an Industrial Strain ofFrontiers in bioengineering and biotechnology · 2019Article
- Combined Drug Resistance Mutations Substantially Enhance Enzyme Production in Paenibacillus agaridevorans.Journal of bacteriology · 2018Article
- Insights into microbial cryptic gene activation and strain improvement: principle, application and technical aspects.The Journal of antibiotics · 2017Review
- Characterization of representative rpoB gene mutations leading to a significant change in toyocamycin production of Streptomyces diastatochromogenes 1628.Journal of industrial microbiology & biotechnology · 2016Article
- Activating the expression of bacterial cryptic genes by rpoB mutations in RNA polymerase or by rare earth elements.Journal of industrial microbiology & biotechnology · 2014Review
- Improvement of daptomycin production in Streptomyces roseosporus through the acquisition of pleuromutilin resistance.BioMed research international · 2013Article
- Article
- New strategies for drug discovery: activation of silent or weakly expressed microbial gene clusters.Applied microbiology and biotechnology · 2013Review
- Combinatorial approaches for inverse metabolic engineering applications.Computational and structural biotechnology journal · 2012Review
- Scandium stimulates the production of amylase and bacilysin in Bacillus subtilis.Applied and environmental microbiology · 2011Article
- Cloning and Expression of Randomly Mutated Bacillus subtilis α-Amylase Genes in HB101.Biotechnology research international · 2011Article
- Identification and characterization of a novel multidrug resistance operon, mdtRP (yusOP), of Bacillus subtilis.Journal of bacteriology · 2009Article
- A novel insertion mutation in Streptomyces coelicolor ribosomal S12 protein results in paromomycin resistance and antibiotic overproduction.Antimicrobial agents and chemotherapy · 2009Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The capacity of ribosomal modification to improve antibiotic production by Streptomyces spp. has already been demonstrated. Here we show that introduction of mutations that produce streptomycin resistance (str) also enhances alpha-amylase (and protease) production by a strain of Bacillus subtilis as estimated by measuring the enzyme activity. The str mutations are point mutations within rpsL, the gene encoding the ribosomal protein S12. In vivo as well as in vitro poly(U)-directed cell-free translation systems showed that among the various rpsL mutations K56R (which corresponds to position 42 in E. coli) was particularly effective at enhancing alpha-amylase production. Cells harboring the K56R mutant ribosome exhibited enhanced translational activity during the stationary phase of cell growth. In addition, the K56R mutant ribosome exhibited increased 70S complex stability in the presence of low Mg2+ concentrations. We therefore conclude that the observed increase in protein synthesis activity by the K56R mutant ribosome reflects increased stability of the 70S complex and is responsible for the increase in alpha-amylase production seen in the affected strain.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.