ArticleJournal of clinical microbiology2006
Development and validation of a rotor-gene real-time PCR assay for detection, identification, and quantification of Chlamydia trachomatis in a single reaction.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 59 citations in OpenAlex.
- Trachoma.Lancet (London, England) · 2025Review
- Highly viable gastrointestinal Chlamydia trachomatis in women abstaining from receptive anal intercourse.Scientific reports · 2025Article
- Molecular Detection ofJournal of arthropod-borne diseases · 2024Article
- Use of viability PCR for detection of live Chlamydia trachomatis in clinical specimens.Frontiers in reproductive health · 2023Article
- Article
- One-step pentaplex real-time polymerase chain reaction assay for detection of zika, dengue, chikungunya, West nile viruses and a human housekeeping gene.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2019Article
- Transcriptional Expression of theMicroorganisms · 2019Article
- Simultaneous detection of eleven sexually transmitted agents using multiplexed PCR coupled with MALDI-TOF analysis.Infection and drug resistance · 2019Article
- A modified method for rapid quantification ofMethodsX · 2019Article
- Simultaneous detection and characterization of toxigenic Clostridium difficile directly from clinical stool specimens.Frontiers of medicine · 2018Article
- Multiplex Strand Invasion Based Amplification (mSIBA) assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae.Scientific reports · 2016Article
- Viability-PCR Shows That NAAT Detects a High Proportion of DNA from Non-Viable Chlamydia trachomatis.PloS one · 2016Article
- Chlamydia trachomatis load in population-based screening and STI-clinics: implications for screening policy.PloS one · 2015Article
- Anorectal Chlamydia trachomatis Load Is Similar in Men Who Have Sex with Men and Women Reporting Anal Sex.PloS one · 2015Article
- Ophthalmic infections in children presenting to Angkor Hospital for Children, Siem Reap, Cambodia.BMC research notes · 2014Article
- Mycoplasma genitalium infection is associated with microscopic signs of cervical inflammation in liquid cytology specimens.Journal of clinical microbiology · 2014Article
- Article
- Generating whole bacterial genome sequences of low-abundance species from complex samples with IMS-MDA.Nature protocols · 2013Article
- Whole-genome sequences of Chlamydia trachomatis directly from clinical samples without culture.Genome research · 2013Article
- Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR.Journal of clinical microbiology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A multitarget real-time PCR (MRT-PCR) for detection of Chlamydia trachomatis DNA was developed and validated. There were three targets for amplification in a single reaction: the cryptic plasmid (CP), the major outer membrane protein (MOMP) gene, and an internal control. The assay had the following characteristics: (i) detection and confirmation of the presence of C. trachomatis DNA in a single reaction, (ii) detection of all genovars of C. trachomatis without any cross-reactivity with pathogenic bacteria or commensal organisms of the oropharynx and genital tract, (iii) a 95% probability of detection with three copies of MOMP and one copy of CP per reaction mixture, (iv) identification of the inhibition of amplification, (v) a quantitative dynamic range of 25 to 250,000 genome copies per reaction mixture, (vi) high intra- and interassay reproducibilities, and (vii) correct identification of all samples in the validation panel. There were 146 COBAS Amplicor PCR (Amplicor PCR)-positive samples and 122 Amplicor PCR-negative samples in the panel. MRT-PCR detected CP DNA alone in 6 (4%) Amplicor PCR-positive samples and both CP and MOMP DNAs in 140 (96%) of 146 Amplicor PCR-positive samples. The quantity of MOMP DNA in 95 (68%) of 140 samples was within the dynamic range of the assay. The median C. trachomatis load in these samples was 321 genome copies per reaction mixture (range, 26 to 40,137 genome copies per reaction mixture). Due to the inclusion of two different C. trachomatis-specific targets, the assay confirmed 259 (97%) of 268 results in a single reaction. This assay could be used in the qualitative format for the routine detection of C. trachomatis and in the quantitative format for study of the pathogenesis of C. trachomatis-associated diseases. The assay demonstrated the potential to eliminate the need for confirmatory testing in almost all samples, thus reducing the turnaround time and the workload.
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