ArticleJournal of clinical microbiology2006
Development and evaluation of a real-time PCR assay targeting the type III secretion system of Burkholderia pseudomallei.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 151 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
151 citing papers in PubMed, 2 syntheses or guidelines pooled it, 226 citations in OpenAlex.
- Whole Genome Sequencing and Comparative Genomics of the Emerging PathogenPathogens (Basel, Switzerland) · 2025Pooled it
- Systematic review and consensus guidelines for environmental sampling of Burkholderia pseudomallei.PLoS neglected tropical diseases · 2013Pooled it
- Successful treatment of pulmonary melioidosis in an Australian dog.Australian veterinary journal · 2026Article
- Throat and rectal swabs to diagnose melioidosis: utility or futility?Journal of clinical microbiology · 2026Article
- Hospital-Based Cross-Sectional Study ofGeoHealth · 2026Article
- CRISPR-based environmental detection of Burkholderia pseudomallei identifies sanitation gaps and melioidosis risk in northeast Thailand.Nature communications · 2026Article
- Current Melioidosis Diagnostic Landscape and Missed Opportunities in Biomarker Development.Diagnostics (Basel, Switzerland) · 2026Review
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- Article
- Environmental Presence of Burkholderia pseudomallei at Military Sites in an Endemic Region: Implications for Future Deployments and Studies.The American journal of tropical medicine and hygiene · 2026Article
- Expanding the molecular epidemiology of melioidosis in North Central Vietnam.PLoS neglected tropical diseases · 2026Article
- Evaluation of culture- and PCR-based methods for detecting Burkholderia pseudomallei in soil samples in Thailand.PLoS neglected tropical diseases · 2026Article
- Laboratory diagnosis of melioidosis.PLoS neglected tropical diseases · 2025Review
- Evidence of populational Burkholderia pseudomallei exposure in Madagascar.PLoS neglected tropical diseases · 2025Article
- Rapidly Progressing Melioidosis Outbreak in City Center Zoo, Hong Kong, 2024.Emerging infectious diseases · 2025Article
- Evaluation of an automated molecular diagnostic instrument for direct detection ofJournal of medical microbiology · 2025Article
- Neuromelioidosis outbreak in Tamil Nadu, India: an investigation of transmission with genomic insights.The Lancet regional health. Southeast Asia · 2025Article
- Point-of-care detection of Burkholderia pseudomallei: A method integrating LAMP and LFSA with RNase HII hydrolysis.PLoS neglected tropical diseases · 2025Article
- Melioidosis cases detected in Dhaka, Bangladesh: a positive impact of 3rd South Asian melioidosis congress-2023.Current research in microbial sciences · 2025Article
- An improved loop mediated isothermal amplification based assay for the rapid identification of genomic DNA ofCurrent research in microbial sciences · 2025Article
91 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we report on the development of a discriminatory real-time assay for the rapid identification of Burkholderia pseudomallei isolates and the evaluation of this assay for sensitivity against related species and detection in spiked human blood samples. The assay targets a 115-base-pair region within orf2 of the B. pseudomallei type III secretion system gene cluster and distinguishes B. pseudomallei from other microbial species. Assay performance was evaluated with 224 geographically, temporally, and clinically diverse B. pseudomallei isolates from the Centers for Disease Control and Prevention strain collection. This represents the first real-time PCR for rapid and sensitive identification of B. pseudomallei that has been tested for cross-reactivity with 23 Burkholderia mallei, 5 Burkholderia thailandensis, and 35 Burkholderia and 76 non-Burkholderia organisms which have historically presented diagnostic challenges. The assay performed with 100% specificity. The limit of detection was found to be 76 femtograms of DNA (equivalent to 5.2 x 10(3) genome equivalents per ml) in a single PCR. In spiked human blood, the assay could detect as few as 8.4 x 10(3) CFU per ml. This rapid assay is a valuable tool for identification of B. pseudomallei and may improve diagnosis in regions endemic for melioidosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.