ArticleThe EMBO journal1992
Cell-specific transcription of the peripherin gene in neuronal cell lines involves a cis-acting element surrounding the TATA box.
Article in The EMBO journal, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 54 citations in OpenAlex.
- Role of the Intermediate Filament Protein Peripherin in Health and Disease.International journal of molecular sciences · 2022Review
- Viral Vector-Based Evaluation of Regulatory Regions in the Neuron-Specific Enolase (NSE) Promoter in Mouse Cerebellum In Vivo.Cerebellum (London, England) · 2017Article
- Intron 1 is required for cell type-specific, but not injury-responsive, peripherin gene expression.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Expression of renin in large arteries outside the kidney revealed by human renin promoter/LacZ transgenic mouse.The American journal of pathology · 2002Article
- A novel basal promoter element is required for expression of the rat tyrosine hydroxylase gene.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Sequences in the proximal 5' flanking region of the rat neuron-specific enolase (NSE) gene are sufficient for cell type-specific reporter gene expression.Journal of molecular neuroscience : MN · 1997Article
- The 66-kDa neurofilament protein (NF-66): sequence analysis and evolution.Neurochemical research · 1996Article
- The JC virus minimal core promoter is glial cell specific in vivo.Journal of virology · 1995Article
- Melanocyte-specific gene expression: role of repression and identification of a melanocyte-specific factor, MSF.Molecular and cellular biology · 1994Article
- Molecular mechanism of transcriptional activation of angiotensinogen gene by proximal promoter.The Journal of clinical investigation · 1994Article
- Binding of TFIID and MEF2 to the TATA element activates transcription of the Xenopus MyoDa promoter.Molecular and cellular biology · 1994Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Peripherin is a neurone-specific intermediate filament protein expressed mostly in the peripheral nervous system. To localize sequences that are important for the regulation of peripherin gene transcription, we have functionally dissected its promoter. Transfection into different cell lines and deletion mapping of peripherin-lacZ hybrid constructs indicated that the first 98 bp preceding the transcription start site of the gene were sufficient to confer cell-type specific expression. DNase I footprinting experiments revealed three protected sequences in this region, that were named PER1, PER2 and PER3. The PER2 and PER3 elements, localized between -98 to -46, interact with proteins that seem widely distributed. Deletion of these elements severely decreased the level of reporter gene activity. The PER1 element, which overlaps the TATA box, interacts with a DNA-binding protein prevailing in peripherin expressing cell lines. However, the core promoter, which contains the PER1 element, was inefficient in driving gene expression. Experiments designed to test the contribution of each element showed that PER2 and PER3 were important in determining the level of expression, while PER1 was important for cell-type specificity. In fact the polyoma virus enhancer linked to the peripherin gene core promoter was found to limit reporter gene activity to peripherin expressing cell lines. Together, these experiments indicate that co-operative interactions between different regions of the promoter are necessary for efficient and cell-type specific transcription of the peripherin gene in a subset of neuronal cells.
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