ArticleGenetics2006
A versatile and efficient gene-targeting system for Aspergillus nidulans.
Article in Genetics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 318 papers.
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Who cites it
318 citing papers in PubMed.
- Article
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- Discovery of a dimeric isopimarane derivative by reexploring a labdane-related diterpenoid biosynthetic gene cluster in Aspergillus nidulans.Microbial cell factories · 2026Article
- Engineering Industrial Strain ofAntibiotics (Basel, Switzerland) · 2026Article
- Functional analysis ofMycoscience · 2026Article
- VapA/Scs2 sustains polarized growth inMicrobial cell (Graz, Austria) · 2026Article
- Acyl-CoA-binding protein AcbdA is required for peroxisome hitchhiking on early endosomes inMolecular biology of the cell · 2025Article
- A genetic strategy to allow detection of F-actin by phalloidin staining in diverse fungi.mSphere · 2025Article
- Systematic over-expression of secondary metabolism transcription factors to reveal the pharmaceutical potential of Aspergillus nidulans.Communications biology · 2025Article
- Fungal Innovations-Advancing Sustainable Materials, Genetics, and Applications for Industry.Journal of fungi (Basel, Switzerland) · 2025Review
- Unique electron transfer system of cytochrome P450 monooxygenase includes a mechanism of fatty acid β-oxidation.Communications biology · 2025Article
- Phenotypic and omics analyses of the SordariomycetesmSystems · 2025Article
- The HOG signal pathway contributes to survival strategies of the piezo-tolerant fungusApplied and environmental microbiology · 2025Article
- The actin module of endocytic internalization in Aspergillus nidulans: A critical role of the WISH/DIP/SPIN90 family protein Dip1.PLoS genetics · 2025Article
- Engineering of Global Transcriptional Regulators (GTRs) inJournal of fungi (Basel, Switzerland) · 2025Review
- A CRISPR Cas12a/Cpf1 strategy to facilitate robust multiplex gene editing in Aspergillus Niger.Fungal biology and biotechnology · 2025Article
- Considerations for Domestication of Novel Strains of Filamentous Fungi.ACS synthetic biology · 2025Review
- Article
- Synthetic biology meets Aspergillus: engineering strategies for next-generation organic acid production.World journal of microbiology & biotechnology · 2025Review
- Co-transformation of Aspergillus fumigatus: a simple and efficient strategy for gene editing without linking selectable markers.Access microbiology · 2025Article
258 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors.
Funding
Abstract
Aspergillus nidulans is an important experimental organism, and it is a model organism for the genus Aspergillus that includes serious pathogens as well as commercially important organisms. Gene targeting by homologous recombination during transformation is possible in A. nidulans, but the frequency of correct gene targeting is variable and often low. We have identified the A. nidulans homolog (nkuA) of the human KU70 gene that is essential for nonhomologous end joining of DNA in double-strand break repair. Deletion of nkuA (nkuA delta) greatly reduces the frequency of nonhomologous integration of transforming DNA fragments, leading to dramatically improved gene targeting. We have also developed heterologous markers that are selectable in A. nidulans but do not direct integration at any site in the A. nidulans genome. In combination, nkuA delta and the heterologous selectable markers make up a very efficient gene-targeting system. In experiments involving scores of genes, 90% or more of the transformants carried a single insertion of the transforming DNA at the correct site. The system works with linear and circular transforming molecules and it works for tagging genes with fluorescent moieties, replacing genes, and replacing promoters. This system is efficient enough to make genomewide gene-targeting projects feasible.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.