ArticleBiophysical journal2006
Characterization of the liquid-ordered state by proton MAS NMR.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 66 citations in OpenAlex.
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- Characterization of lipid rafts in human platelets using nuclear magnetic resonance: A pilot study.Biochemistry and biophysics reports · 2017Article
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- Cholesterol-induced suppression of membrane elastic fluctuations at the atomistic level.Chemistry and physics of lipids · 2016Review
- Measuring selective estrogen receptor modulator (SERM)-membrane interactions with second harmonic generation.Journal of the American Chemical Society · 2014Article
- Minimal effect of lipid charge on membrane miscibility phase behavior in three ternary systems.Biophysical journal · 2013Article
- Recombinant cannabinoid type 2 receptor in liposome model activates g protein in response to anionic lipid constituents.The Journal of biological chemistry · 2012Article
- Phase-separation and domain-formation in cholesterol-sphingomyelin mixture: pulse-EPR oxygen probing.Biophysical journal · 2011Article
- High-throughput screening of drug-lipid membrane interactions via counter-propagating second harmonic generation imaging.Analytical chemistry · 2011Article
- Acyl-chain methyl distributions of liquid-ordered and -disordered membranes.Biophysical journal · 2011Article
- Cholesterol and lipid phases influence the interactions between serotonin receptor agonists and lipid bilayers.The Journal of biological chemistry · 2010Article
- Phase separation in binary mixtures of bipolar and monopolar lipid dispersions revealed by 2H NMR spectroscopy, small angle x-ray scattering, and molecular theory.Biophysical journal · 2009Article
- Liquid ordered and gel phases of lipid bilayers: fluorescent probes reveal close fluidity but different hydration.Biophysical journal · 2008Article
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
We investigated if magic angle spinning (MAS) 1H NMR can be used as a tool for detection of liquid-ordered domains (rafts) in membranes. In experiments with the lipids SOPC, DOPC, DPPC, and cholesterol we demonstrated that 1H MAS NMR spectra of liquid-ordered domains (lo) are distinctly different from liquid-disordered (ld) and solid-ordered (so) membrane regions. At a MAS frequency of 10 kHz the methylene proton resonance of hydrocarbon chains in the ld phase has a linewidth of 50 Hz. The corresponding linewidth is 1 kHz for the lo phase and several kHz for the so phase. According to results of 1H NMR dipolar echo spectroscopy, the broadening of MAS resonances in the lo phase results from an increase in effective strength of intramolecular proton dipolar interactions between adjacent methylene groups, most likely because of a lower probability of gauche/trans isomerization in lo. In spectra recorded as a function of temperature, the onset of lo domain (raft) formation is seen as a sudden onset of line broadening. Formation of small domains yielded homogenously broadened resonance lines, whereas large lo domains (diameter >0.3 microm) in an ld environment resulted in superposition of the narrow resonances of the ld phase and the much broader resonances of lo. 1H MAS NMR may be applied to detection of rafts in cell membranes.
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